Repeated anodal transcranial direct current stimulation induces neural plasticity-associated gene expression in the rat cortex and hippocampus
Repeated anodal transcranial direct current stimulation induces neural plasticity-associated gene expression in the rat cortex and hippocampus
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DOI：
10.3233/rnn-160689
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发表时间：
2017-01-01
影响因子：
2.8
通讯作者：
Shin, Yong-Il
中科院分区：
文献类型：
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作者：
Kim, Min Sun;Koo, Ho;Shin, Yong-Il
背景资料：阳极经颅直流电刺激（Anodal transcranial direct current stimulation，A-tDCS）可诱导皮层兴奋性的长期增加，从而增加脑内基因转录。目的：本研究的目的是评估A-tDCS后幼龄Sprague-Dawley大鼠感觉运动皮层和海马中活动依赖性神经元可塑性相关基因的表达。我们应用A-tDCS在右侧感觉运动皮层epicranial与圆形电极（3 mm直径）在250 μ A，每天20分钟，连续7天。使用SYBR绿色定量真实的-时间聚合酶链反应（PCR）分析脑源性神经营养因子（BDNF）、cAMP反应元件结合蛋白（CREB）、突触蛋白I、Ca 2 +/钙调蛋白依赖性蛋白激酶II（CaMKII）、活性调节的细胞因子相关蛋白（Arc）和c-Fos的mRNA水平。我们发现，单侧A-tDCS 7天导致同侧皮质中所有可塑性相关基因的转录显著增加。每日A-tDCS也导致c-Fos mRNA在同侧hippocamps.Conclusion的显着增加：这些结果表明，在皮层和海马可塑性相关基因的表达改变是A-tDCS诱导的神经可塑性的分子底物。
Background: Anodal transcranial direct current stimulation (A-tDCS) induces a long- lasting increase in cortical excitability that can increase gene transcription in the brain.Objective: The purpose of this study was to evaluate the expression of genes related to activity-dependent neuronal plasticity in the sensorimotor cortex and hippocampus of young Sprague-Dawley rats following A-tDCS.Methods: We applied A-tDCS over the right sensorimotor cortex epicranially with a circular electrode (3mm diameter) at 250 mu A for 20 min per day for 7 consecutive days. Levels of mRNA for brain-derived neurotrophic factor (BDNF), cAMP response element-binding protein (CREB), synapsin I, Ca2+/calmodulin-dependent protein kinase II (CaMKII), activity-regulated cytoskeleton-associated protein (Arc), and c-Fos were analyzed using SYBR Green quantitative real- time polymerase chain reaction (PCR).Results: We found that 7 days of unilateral A-tDCS resulted in significant increases in transcription of all plasticity-related genes tested in the ipsilateral cortex. Daily A-tDCS also resulted in a significant increase in c-Fos mRNA in the ipsilateral hippocampus.Conclusion: These results indicate that altered expression of plasticity-associated genes in the cortex and hippocampus is a molecular substrate of A-tDCS-induced neural plasticity.