Seeing the Portal in Herpes Simplex Virus Type 1 B Capsids
Seeing the Portal in Herpes Simplex Virus Type 1 B Capsids
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DOI：
10.1128/jvi.01663-10
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Chiu, W.
中科院分区：
文献类型：
--
作者：
Rochat, R. H.;Liu, X.;Chiu, W.
解析大型二十面体病毒中的非二十面体组分仍然是结构病毒学的技术挑战。我们已经使用了新兴的泽尼克相衬电子冷冻显微镜技术，以提高冰包埋的单纯疱疹病毒1型衣壳的图像对比度。图像重建使我们能够检索到的独特的门户顶点的结构的上下文中的二十面体衣壳，并首次显示的亚基组织的门户感染真核生物的病毒。我们的地图明确地解决了12亚基门户网站位于五聚体顶点之一，从而消除了不确定性的疱疹病毒门户网站的位置和化学计量。
Resolving the nonicosahedral components in large icosahedral viruses remains a technical challenge in structural virology. We have used the emerging technique of Zernike phase-contrast electron cryomicroscopy to enhance the image contrast of ice-embedded herpes simplex virus type 1 capsids. Image reconstruction enabled us to retrieve the structure of the unique portal vertex in the context of the icosahedral capsid and, for the first time, show the subunit organization of a portal in a virus infecting eukaryotes. Our map unequivocally resolves the 12-subunit portal situated beneath one of the pentameric vertices, thus removing uncertainty over the location and stoichiometry of the herpesvirus portal.