Class 1 integron-borne, multiple-antibiotic resistance encoded by a 150-kilobase conjugative plasmid in epidemic Vibrio cholerae O1 strains isolated in Guinea-Bissau
Class 1 integron-borne, multiple-antibiotic resistance encoded by a 150-kilobase conjugative plasmid in epidemic Vibrio cholerae O1 strains isolated in Guinea-Bissau
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DOI：
10.1128/jcm.38.10.3774-3779.2000
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发表时间：
2000-10-01
影响因子：
9.4
通讯作者：
Sandström, A
中科院分区：
文献类型：
--
作者：
Dalsgaard, A;Forslund, A;Sandström, A
1996-1997年几内亚比绍霍乱流行病。对抗菌素耐药性的监测表明，在流行过程中出现了霍乱弧菌O 1的多重耐药性菌株。该菌株对青霉素、红霉素、四环素耐药？呋喃唑酮、氨基糖苷类、甲氧苄啶和磺胺甲恶唑。伴随着这种菌株的出现，我们观察到登记的霍乱病例数量出现回升，因为病死率从多重耐药菌株出现前的1.0%增加到菌株出现后的5.3%。我们的研究表明，该菌株含有一个150 kb的接合性多重抗生素耐药质粒，该质粒具有编码对甲氧苄啶（dhfrXII）和氨基糖苷类抗生素耐药的1类整合子携带的基因盒[ant（3“-1a]）。发现对几乎所有通常用于治疗霍乱的抗生素都具有可转移的耐药性，这是一个重大的公共卫生问题。应进行研究，以确定该菌株或其耐药性基因在多大程度上已传播到霍乱流行的其他地区。
in the 1996-1997 cholera epidemic in Guinea-Bissau. surveillance for antimicrobial resistance showed the emergence of a multidrug-resistant strain of Vibrio cholerae O1 during the course of the epidemic. The strain was resistant to ampicillin, erythromycin, tetracycline? furazolidone, aminoglycosides, trimethoprim, and sulfamethoxazole. Concomitant with the emergence of this strain, we observed a resurgence in the number of registered cholera cases as web as an increase in the case fatality rate from 1.0% before the emergence of the multiple-drug-resistant strain to 5.3% after the emergence of the strain. Our study shows that the strain contained a 150-kb conjugative multiple-antibiotic resistance plasmid with class 1 integron-borne gene cassettes encoding resistance to trimethoprim (dhfrXII) and aminoglycosides [ant(3 "-1a]). The finding of transferable resistance to almost all of the antibiotics commonly used to treat cholera is of great public health concern. Studies should be carried out to determine to what extent the strain or its resistance genes have been spread to other areas where cholera is endemic.