The jasmonate-ZIM domain gene VqJAZ4 from the Chinese wild grape Vitis quinquangularis improves resistance to powdery mildew in Arabidopsis thaliana.
The jasmonate-ZIM domain gene VqJAZ4 from the Chinese wild grape Vitis quinquangularis improves resistance to powdery mildew in Arabidopsis thaliana.
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DOI：
10.1016/j.plaphy.2019.09.018
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
Guofeng Zhang;Xiaoxiao Yan;Songlin Zhang;Yanxun Zhu;Xiuming Zhang;Hengbo Qiao;S. van Nocker;
中科院分区：
文献类型：
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作者：
Guofeng Zhang;Xiaoxiao Yan;Songlin Zhang;Yanxun Zhu;Xiuming Zhang;Hengbo Qiao;S. van Nocker;
葡萄（葡萄属vinifera L.）是最广泛种植和经济上重要的水果之一。大多数栽培的葡萄品种极易感染真菌疾病，其中最普遍的是由Uncinulanecator引起的白粉病。茉莉酸-ZIM结构域（JAZ）家族蛋白在植物对环境胁迫的响应中起着关键作用。在这里，我们报告的VqJAZ 4，茉莉酸ZIM结构域基因分离的毛葡萄，中国野生葡萄种，表现出高耐受性的几种真菌。亚细胞定位实验表明VqJAZ 4蛋白定位于细胞核。U. necatorinoculation，以及防御相关的激素茉莉酸甲酯（MeJA）和水杨酸（SA）。接种后VqJAZ 4的表达上调依赖于其启动子序列。VqJAZ 4基因在拟南芥中的表达提高了对白粉病的抗性。组织化学染色结果表明，表达VqJAZ 4的植株比非转基因对照（NTC）植株表现出更多的死亡细胞和更强的活性氧（ROS）爆发。对几个疾病相关基因的表达分析表明，VqJAZ 4的表达通过SA和/或JA信号途径增强了防御反应。我们还发现表达VqJAZ 4的拟南芥对灰葡萄孢的易感性增加。这些结果表明，VqJAZ 4可能在葡萄对病原真菌的应答中发挥重要作用，并可能成为未来葡萄抗病分子育种的候选基因。
Grape (Vitis vinifera L.) is one of the most widely cultivated and economically important fruits. Most cultivated varieties of grape are highly susceptible to fungal diseases, and one of the most pervasive is powdery mildew, caused byUncinulanecator. The jasmonate-ZIM domain (JAZ) family proteins are critical for plant responses to environmental stresses. Here, we report the characterization ofVqJAZ4, a jasmonate-ZIM domain gene isolated fromVitisquinquangularis, a Chinese wildVitisspecies that exhibits high tolerance to several kinds of fungi. Subcellular localization assay indicated that the VqJAZ4 protein is targeted to the nucleus. TheVqJAZ4gene was strongly induced byU. necatorinoculation, as well as by the defense-related hormones methyl jasmonate (MeJA) and salicylic acid (SA). The upregulation ofVqJAZ4after inoculation was dependent on its promoter sequences. Expression ofVqJAZ4in Arabidopsis thaliana improved resistance to powdery mildew. Histochemical staining assays indicated that plants expressingVqJAZ4displayed a larger number of dead cells and stronger reactive oxygen species (ROS) burst than non-transgenic control (NTC) plants. Expression analysis of several disease-related genes suggested thatVqJAZ4expression enhanced defense responses though SA and/or JA signaling pathways. We also found thatVqJAZ4-expressing Arabidopsis showed increased susceptibility to Botrytis cinerea. Taken together, these results provide evidence thatVqJAZ4may play an important role in response to fungal pathogens in grape, and may represent a candidate for future grape molecular breeding for disease resistance.