Wet-Chemical Enzymatic Preparation and Characterization of Ultrathin Gold-Decorated Single Glass Nanopore
Wet-Chemical Enzymatic Preparation and Characterization of Ultrathin Gold-Decorated Single Glass Nanopore
复制标题
超薄金修饰单玻璃纳米孔的湿化学酶法制备及表征
DOI：
10.1021/ac404168s
复制
发表时间：
2014-05-20
影响因子：
7.4
通讯作者：
Jin, Yongdong
中科院分区：
文献类型：
--
作者：
He, Haili;Xu, Xiaolong;Jin, Yongdong
通过静电沉积葡萄糖氧化酶单层膜对锥形玻璃纳米孔进行修饰，然后通过酶催化反应原位形成纳米金膜。利用透射电子显微镜（TEM）和能量色散X射线（EDX）分析分别表征了单玻璃纳米孔沉积Au前后的形貌和成分。特别地，纳米孔尖端区中量子点荧光的淬灭进一步说明金纳米膜成功地沉积在单个玻璃纳米孔的内壁上。Au薄膜使玻璃纳米孔更生物友好，并允许纳米孔通过Au S键容易地功能化表面。例如，通过引入不同的硫醇分子，可以在不同的pH下容易地切换金装饰的玻璃纳米孔的离子电流整流（ICR）特性。
The conical glass nanopore was modified through layer-by-layer electrostatic deposition of a monolayer of glucose oxidase, and then an ultrathin gold film was formed in situ through enzyme-catalyzed reactions. The morphology and components of single glass nanopore before and after ultrathin Au deposition were characterized by transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX) analysis, respectively. In particular, the quenching of the quantum dots fluorescence in the nanopore tip zone further illustrated that the gold nanofilm was successfully deposited on the inner wall of the single glass nanopore. The Au thin films make the glass nanopores more biologically friendly and allow the nanopores facile functionalization of the surface through the Au S bonds. For instance, the ionic current rectification (ICR) properties of the gold-decorated glass nanopores could be switched readily at different pHs by introducing different thiol molecules.