Natural killer cell function in human lung is compartmentalized.
Natural killer cell function in human lung is compartmentalized.
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人肺中的自然杀伤细胞功能是分开的。
DOI：
10.1164/arrd.1987.135.4.941
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Toews,GB
中科院分区：
文献类型：
--
作者：
Weissler,JC;Nicod,LP;Lipscomb,MF;Toews,GB
自然杀伤（NK）细胞是能够杀死多种肿瘤靶标的淋巴细胞亚群。NK细胞在动物模型中可以限制肺转移，并且可能是人肺肿瘤防御的重要介质。然而，先前的人类研究表明，通过支气管肺泡灌洗（BAL）获得的肺NK细胞在功能上是惰性的。我们从人肺组织中纯化了淋巴细胞，并将这些细胞的NK活性与从外周血中纯化的淋巴细胞进行了比较。从切碎的全肺标本中获得的NK细胞的活性与外周血中发现的活性相当。用抗外周血大颗粒淋巴细胞（LGL）上2个标志物的单克隆抗体测定肺NK细胞表型。表达Leu 11b的淋巴细胞负责肺NK活性，因为抗Leu 11b和补体预处理可消除所有NK功能。与此相反，淋巴细胞表达Leu 7几乎没有NK活性。尽管血液中表达Leu 7或Leu 11的淋巴细胞的频率相当，但肺中Leu 7+淋巴细胞显著多于Leu 11+细胞（p < 0.001）。肺组织的免疫荧光染色显示，表达Leu 11的LGL主要局限于肺小叶，而表达Leu 7的LGL则经常位于肺泡和较大的气道。这些结果表明，NK功能是存在于人肺，它是区室化的NK活性Leu 11+淋巴细胞主要位于肺结节。
Natural killer (NK) cells are a subpopulation of lymphocytes capable of killing a variety of neoplastic targets. NK can limit pulmonary metastases in animal models and could be important mediators of tumor defense in human lung. Previous studies in humans have suggested, however, that pulmonary NK cells obtained by bronchoalveolar lavage (BAL) are functionally inert. We purified lymphocytes from human lung tissue and compared the NK activity of these cells with lymphocytes purified from peripheral blood. The activity of NK cells obtained from minced whole lung specimens was comparable to that found in peripheral blood. Monoclonal antibodies to 2 markers found on peripheral blood large granular lymphocytes (LGL) were utilized to determine the phenotype of pulmonary NK cells. Lymphocytes expressing Leu 11b were responsible for pulmonary NK activity as all NK function was abrogated by pretreatment with anti-Leu 11b and complement. In contrast, lymphocytes expressing Leu 7 had little NK activity. Although the frequency of lymphocytes expressing Leu 7 or Leu 11 in blood was comparable, there were significantly more (p < 0.001) Leu 7+ lymphocytes in the lung than Leu 11+ cells. Immunofluorescent staining of lung tissue demonstrated that LGL expressing Leu 11 were confined largely to the lung interstitium, whereas LGL expressing Leu 7 were frequently located in alveoli and larger airways. These findings suggest that NK function is present in the human lung and that it is compartmentalized with the NK-active Leu 11+ lymphocytes located primarily in the lung interstitium.