The genome of the Pseudomonas aeruginosa generalized transducing bacteriophage F116
The genome of the Pseudomonas aeruginosa generalized transducing bacteriophage F116
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DOI：
10.1016/j.gene.2004.11.001
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发表时间：
2005-02-14
期刊：
影响因子：
3.5
通讯作者：
Kropinski, AM
中科院分区：
文献类型：
--
作者：
Byrne, M;Kropinski, AM
F116 是一种温带、菌毛特异性、通用转导噬菌体，属于 Podoviridae 病毒家族。其基因组为线性、ds、TR、CP DNA，GC含量为63.2%。 65 195 bp 基因组包含 70 个假定的 ORF，其中只有 16 个显示出与假单胞菌基因组或噬菌体基因的序列相似性。虽然当前文献表明 F116 是一种非整合噬菌体，在溶原生命周期中将自身维持为质粒，但鉴定出了假定的 int 基因。在噬菌体结构基因中，只有门户蛋白可以通过同源性鉴定。通过一维 SDS-PAGE 对 F116 结构蛋白的分析显示大约 15 个条带。 MALDI-TOF MS 分析鉴定了编码主要衣壳蛋白的基因。该蛋白质似乎经历翻译后裂解，产生更小的衣壳蛋白。 (c) 2004 年，Elsevier B.V. 出版
F116 is a temperate, pilus-specific, generalized transducing phage belonging to the Podoviridae virus family. Its genome is linear, ds, TR, and CP DNA with a GC content of 63.2%. The 65 195-bp genome contains 70 putative ORFs, only 16 of which showed sequence similarity to Pseudomonas genomic or phage genes.While the current literature suggests that F116 is a non-integrating phage that maintains itself as a plasmid during the lysogenic life cycle, a putative int gene was identified. Of the phage structural genes, only the portal protein could be identified by homology. Analysis of F116 structural protein by one-dimensional SDS-PAGE revealed approximately 15 bands. MALDI-TOF MS analysis identified the gene encoding the major capsid protein. This protein appears to undergo posttranslational cleavage giving rise to a smaller capsid protein. (c) 2004 Published by Elsevier B.V.