Collagen binding by the mannose receptor mediated through the fibronectin type 11 domain
Collagen binding by the mannose receptor mediated through the fibronectin type 11 domain
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DOI：
10.1042/bj20052027
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发表时间：
2006-05-01
影响因子：
4.1
通讯作者：
Taylor, ME
中科院分区：
文献类型：
--
作者：
Napper, CE;Drickamer, K;Taylor, ME
巨噬细胞甘露糖受体是受体家族的原型，每个受体具有由与蓖麻毒素的R型碳水化合物识别结构域相关的所有N-末端富含半胱氨酸的结构域、纤连蛋白II型结构域和与C型碳水化合物识别结构域相关的八至十个结构域组成的胞外区。甘露糖受体作为一种分子清除剂，通过识别其定义的碳水化合物结构来清除有害的糖缀合物或微生物。细胞粘附试验，以及胶原蛋白结合试验，现在已经被用来表明，甘露糖受体也可以结合胶原蛋白和纤连蛋白II型结构域介导这种活动。受体中两种类型的糖结合结构域都不参与胶原结合。表达甘露糖受体的成纤维细胞粘附于I型、III型和IV型胶原，但不粘附于V型胶原，并且粘附被分离的甘露糖受体纤连蛋白II型结构域抑制。纤连蛋白II型结构域显示与整个受体相同的胶原特异性，结合I型、III型和IV型胶原。这是甘露糖受体的纤连蛋白II型结构域的第一个活性。结果表明，这种多功能受体在介导胶原清除或细胞-基质粘附中的额外作用。
The macrophage mannose receptor is the prototype for a family of receptors each having an extracellular region consisting of all N-terminal cysteine-rich domain related to the R-type carbohydrate-recognition domain of ricin, a fibronectin type II domain and eight to ten domains related to C-type carbohydrate-recognition domains. The mannose receptor acts as a molecular scavenger, clearing harmful glycoconjugates or micro-organisms through recognition of their defining carbohydrate structures. Cell-adhesion assays, as well as collagen-binding assays, have now been used to show that the mannose receptor can also bind collagen and that the fibronectin type II domain mediates this activity. Neither of the two types of sugar-binding domain in the receptor is involved in collagen binding. Fibroblasts expressing the mannose receptor adhere to type I, type III and type IV collagens, but not to type V collagen, and the adherence is inhibited by isolated mannose receptor fibronectin type II domain. The fibronectin type II domain shows the same specificity for collagen as the whole receptor, binding to type I, type III and type IV collagens. This is the first activity assigned to the fibronectin type It domain of the mannose receptor. The results suggest additional roles for this multifunctional receptor in mediating collagen clearance or cell-matrix adhesion.