Reduction of human immunodeficiency virus-infected cells from donor blood by leukocyte filtration.
Reduction of human immunodeficiency virus-infected cells from donor blood by leukocyte filtration.
复制标题
通过白细胞过滤减少供体血液中受人类免疫缺陷病毒感染的细胞。
DOI：
10.1046/j.1537-2995.1989.29589284150.x
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Vyas,GN
中科院分区：
文献类型：
--
作者：
Rawal,BD;Busch,MP;Endow,R;Garcia-de-Lomas,J;Perkins,HA;Schwadron,R;Vyas,GN
对几种去除白细胞的过滤器进行了评估，评估了它们降低血液单位中细胞相关人类免疫缺陷病毒(HIV)载量的能力，这些血液单位是用来自慢性感染细胞系的淋巴细胞接种在体外的，或者直接从血清阳性捐赠者那里采集的。过滤实验接种的血液单位导致组织培养感染单位(TCIU)的平均减少5.9log10(95%可信区间：7.4-4.5)，通过球菌培养试验的终点滴定进行测定。通过共培养和聚合酶链式反应(PCR)技术检测，对抗HIV阳性献血者的单位血液进行过滤后，传染性降低了2个对数以上。然而，在大多数实验中都检测到了残留的细胞相关病毒。有必要进行临床研究，以确定血液中的白细胞过滤是否会降低输血传播病毒感染的风险。
Several filters for leukocyte removal were evaluated in terms of their ability to reduce the cell‐associated human immunodeficiency virus (HIV) load in units of blood either inoculatedin vitrowith lymphocytes from a chronically infected cell line or collected directly from seropositive donors. Filtration of the experimentally inoculated units of blood resulted in a 5.9 log10mean reduction (95% confidence interval:7.4–4.5) of tissue culture infectious units (TCIU) as assayed by end‐point titration using the cocculture assay. Filtration of the units of blood from anti‐HIV positive donors lowered the infectivity by over 2 logs, as detected by the coculture and polymerase chain reaction (PCR) techniques. However, residual cell‐associated virus was detected in the majority of experiments. Clinical studies are warranted to determine if leukocyte filtration of blood will reduce the risk of transfusion transmitted viral infections.