RIBONUCLEOPROTEIN OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
RIBONUCLEOPROTEIN OF AVIAN INFECTIOUS-BRONCHITIS VIRUS
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DOI：
10.1099/0022-1317-53-1-67
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发表时间：
1981-01-01
影响因子：
3.8
通讯作者：
MACNAUGHTON, MR
中科院分区：
文献类型：
--
作者：
DAVIES, HA;DOURMASHKIN, RR;MACNAUGHTON, MR
本文报道了鸡传染性支气管炎病毒（IBV）核糖核蛋白（RNP）的碳/铂显色电镜观察。来自三个IBV毒株的长度达6.7 µm的线性RNP链对胰腺RNA酶和蛋白酶都敏感。这些链是从自发破裂的完整颗粒获得的，但不是从缺乏RNP的破裂的不完整颗粒获得的。它们也从Nonidet P40破坏的颗粒中释放出来，并且可以在密度为1.27 g/ml的蔗糖密度梯度上分离。在某些情况下，观察到与病毒颗粒相关的螺旋RNP复合物与人冠状病毒株229 E和小鼠肝炎病毒株3的RNP相似。
The ribonucleoprotein (RNP) of avian infectious bronchitis virus (IBV) was examined by electron microscopy after shadowing with carbon/platinum. Linear RNP strands up to 6.7 µm in length, from three IBV strains, were sensitive to both pancreatic RNase and to proteases. These strands were obtained from spontaneously disrupted complete particles but not from disrupted incomplete particles that lacked RNP. They were also released from Nonidet P40-disrupted particles and could be isolated on sucrose density gradients at a density of 1.27 g/ml. In some cases, helical RNP complexes associated with virus particles were observed that were similar to RNPs of human coronavirus strain 229E and mouse hepatitis virus strain 3.