Comparison of serum biochemical parameters between two broiler chicken lines divergently selected for abdominal fat content
Comparison of serum biochemical parameters between two broiler chicken lines divergently selected for abdominal fat content
复制标题
腹部脂肪含量不同选择的两个肉鸡品系之间血清生化参数的比较。
DOI：
10.2527/jas.2015-8871
复制
发表时间：
2015-07-01
影响因子：
3.3
通讯作者：
Li, H.
中科院分区：
文献类型：
--
作者：
Dong, J. -Q.;Zhang, H.;Li, H.
在人类中，肥胖与血清生化指标水平的升高或降低有关。然而，这种关系在鸡中并不清楚。由于长期对快速生长速度的强烈选择，现代肉鸡存在过度脂肪沉积的问题，表现出生化或代谢变化。本研究以东北农业大学肉鸡腹脂率差异选择品系（NEAUHLF）为试验材料，研究了2个品系间血清生化指标的差异。对东北大耳白对虾第16、17、18代群体的18项血清生化指标进行了调查，并对这些血清生化指标的遗传参数进行了估计。对3个世代的数据进行综合分析，结果表明：16项血清生化指标在瘦肉型和肥育型之间存在显著差异。肥胖鸡血清高密度脂蛋白胆固醇（HDL-C）、HDL-C：低密度脂蛋白胆固醇（LDL-C）、总胆汁酸、总蛋白、白蛋白、球蛋白、天冬氨酸转氨酶（AST）：丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（GGT）、尿酸和肌酐浓度极显著高于瘦鸡（P < 0.01），而LDL-C、白蛋白：球蛋白、葡萄糖、AST、ALT和游离脂肪酸浓度极显著低于瘦鸡（P < 0.01）。在这16项血清生化指标中，5项（LDL-C、HDL-C：LDL-C、总胆汁酸、白蛋白和白蛋白：球蛋白）具有高遗传力（0.58 ≤ h2 ≤ 0.89），6项（HDL-C、总蛋白、球蛋白、AST：ALT、GGT和肌酐）具有中等遗传力（0.29 ≤ h2 ≤ 0.48），其余5项具有低遗传力（h2 < 0.20）。血清HDL-C、HDL-C：LDL-C、血糖与腹脂性状的遗传相关系数（rg）为0.30 ≤ rg ≤ 0.80，而血清球蛋白、AST、尿酸与腹脂性状的遗传相关系数为-0.62 ≤ rg ≤ -0.30。其余10项生化指标与腹脂性状的遗传相关较低（-0.30总之，我们确定血清HDL-C和HDL-C：LDL-C水平作为选择瘦肉型家禽的潜在生物标志物。这些发现也将在未来的研究中用于调查人类以及其他动物物种的肥胖和脂质代谢。
In humans, obesity is associated with increased or decreased levels of serum biochemical indicators. However, the relationship is not as well understood in chickens. Due to long-term intense selection for fast growth rate, modern broilers have the problem of excessive fat deposition, exhibiting biochemical or metabolic changes. In the current study, the Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used to identify differences in serum biochemical parameters between the 2 lines. A total of 18 serum biochemical indicators were investigated in the 16th, 17th, and 18th generation populations of NEAUHLF, and the genetic parameters of these serum biochemical indicators were estimated. After analyzing the data from these 3 generations together, the results showed that the levels of 16 of the tested serum biochemical parameters were significantly different between the lean and fat birds. In the fat birds, serum concentrations of high-density lipoprotein cholesterol (HDL-C), HDL-C:low-density lipoprotein cholesterol (LDL-C), total bile acid, total protein, albumin, globulin, aspartate transaminase (AST):alanine transaminase (ALT), γ-glutamyl transpeptidase (GGT), uric acid, and creatinine were very significantly higher (P < 0.01), whereas LDL-C, albumin:globulin, glucose, AST, ALT, and free fatty acids concentrations in serum were very significantly lower than those in the lean birds (P < 0.01). Of these 16 serum biochemical parameters, 5 (LDL-C, HDL-C:LDL-C, total bile acid, albumin, and albumin:globulin) had high heritabilities (0.58 ≤ h2 ≤ 0.89), 6 (HDL-C, total protein, globulin, AST:ALT, GGT, and creatinine) had moderate heritabilities (0.29 ≤ h2 ≤ 0.48), and the remaining 5 had low heritabilities (h2 < 0.20). Serum HDL-C, HDL-C:LDL-C, and glucose had higher positive genetic correlation coefficients (rg) with abdominal fat traits (0.30 ≤ rg ≤ 0.80), whereas serum globulin, AST, and uric acid showed higher negative genetic correlations with abdominal fat traits (–0.62 ≤ rg ≤ –0.30). The remaining 10 serum biochemical parameters had lower genetic correlations with abdominal fat traits (–0.30 < rg < 0.30). In conclusion, we identified serum HDL-C and HDL-C:LDL-C levels as potential biomarkers for selection of lean birds. These findings will also be useful in future studies for investigating obesity and lipid metabolism in humans as well as in other animal species.