Vascular endothelial growth factor modulates contractile response in microvascular lung pericytes
Vascular endothelial growth factor modulates contractile response in microvascular lung pericytes
复制标题
DOI：
10.1016/j.amjsurg.2005.10.034
复制
发表时间：
2006-03-01
影响因子：
3
通讯作者：
Wilson, RF
中科院分区：
文献类型：
--
作者：
Donoghue, L;Tyburski, JG;Wilson, RF
背景：周细胞是一种毛细血管支持细胞，可能通过其收缩反应发挥调节通透性的作用。血管内皮生长因子(VEGF)可能在脓毒症和其他炎症条件下的通透性增加中发挥作用。本研究的目的是探讨血管内皮生长因子对周细胞收缩的调节作用。方法：按上述方法分离大鼠肺微血管周细胞，在胶原凝胶基质上培养。细胞暴露于不同时间段(0、10、30、60和120分钟)的血管内皮生长因子(10、100和1000pg/mL)。结果：在所有剂量下，血管内皮生长因子均可诱导周细胞松弛(收缩幅度为对照组的85%~90%，P<；.001)。随之而来的是增加的和持续的收缩(对照组的107%到120%；P<；0.01)。结论：血管内皮生长因子改变了微血管肺周细胞的收缩反应。这一机制可能在炎症状态下表现出的通透性增加中起作用。(C)2006 Excerpta Medica Inc.保留所有权利。
Background: Pericytes are capillary support cells that may play a role in regulating permeability by their contractile responses. Vascular endothelial growth factor (VEGF) may play a role in the increased permeability found in sepsis and other inflammatory conditions. The purpose of this study was to evaluate the role of VEGF in regulating pericyte contraction.Methods: Rat microvascular lung pericytes were isolated according to previously described methods and cultured on collagen gel matrices. Cells were exposed to VEGF (10, 100, and 1000 pg/mL) for varying time periods (0, 10, 30, 60, and 120 minutes). The gels were released and their contractile responses digitally quantified.Results: At all doses, VEGF induced initial pericyte relaxation (contraction 85% to 90% of controls;, P < .001). This was followed-up by increased and sustained contraction (107% to 120% of controls; P < .01).Conclusions: VEGF modifies the contractile response of microvascular lung pericytes. This mechanism may play a role in the increased permeability demonstrated in inflammatory states. (c) 2006 Excerpta Medica Inc. All rights reserved.