Monodisperse Uni- and Multicompartment Liposomes
Monodisperse Uni- and Multicompartment Liposomes
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DOI：
10.1021/jacs.6b02107
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发表时间：
2016-06-22
影响因子：
15
通讯作者：
Huck, Wilhelm T. S.
中科院分区：
文献类型：
--
作者：
Deng, Nan-Nan;Yelleswarapu, Maaruthy;Huck, Wilhelm T. S.
脂质体是一种自组装的磷脂囊泡，在从靶向给药到人工细胞等领域具有巨大的应用潜力。使用微流体技术形成脂质体已经取得了相当大的进展，但是脂质体形成过程本身还没有被非常详细地研究。因此，高通量、高产率的多室单分散脂质体制备方法尚未得到证实。在这里，我们报告的表面活性剂辅助的微流体路线均匀，单双层脂质体，范围从25至190 itm，并有或没有多个内部隔室。我们的方法的关键是精确控制复杂的W/O/W乳液体系在脂质体形成过程中的界面能，这是通过在外水相中添加表面活性剂来实现的。脂质体由单个双层组成，如纳米孔形成实验和共聚焦荧光显微镜所示，它们可以作为无细胞基因表达的隔室。微流体技术可以扩展到创建具有多个耦合隔室的脂质体，从而打开通向多步微反应器网络的路线。
Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from targeted drug delivery to artificial cells. The formation of liposomes using microfluidic techniques has seen considerable progress, but the liposomes formation process itself has not been studied in great detail. As a result, high throughput, high-yielding routes to monodisperse liposomes with multiple compartments have not been demonstrated. Here, we report on a surfactant-assisted microfluidic route to uniform, single bilayer liposomes, ranging from 25 to 190 itm, and with or without multiple inner compartments. The key of our method is the precise control over the developing interfacial energies of complex W/O/W emulsion systems during liposome formation, which is achieved-via an additional surfactant in the outer water phase. The liposomes consist of single bilayers, as demonstrated by nanopore formation experiments and confocal fluorescence microscopy, and they can act as compartments for cell-free gene expression. The microfluidic technique can be expanded to create liposomes with a multitude of coupled compartments, opening routes to networks of multistep microreactors.