A novel B cell population revealed by a CD38/CD24 gating strategy: CD38-CD24- B cells in centenarian offspring and elderly people
A novel B cell population revealed by a CD38/CD24 gating strategy: CD38-CD24- B cells in centenarian offspring and elderly people
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DOI：
10.1007/s11357-012-9488-5
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发表时间：
2013-10-01
期刊：
影响因子：
--
通讯作者：
Colonna-Romano, Giuseppina
中科院分区：
文献类型：
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作者：
Buffa, Silvio;Pellicano, Mariavaleria;Colonna-Romano, Giuseppina
适应性免疫的B细胞臂随着年龄的增长而发生显著的变化。老年人的特征是B细胞反应受损，反映在有效对抗病毒和细菌的能力降低。随着年龄的增长，免疫力的变化（免疫衰老）已经在百岁老人中得到了广泛的研究，他们被认为是成功衰老的一个很好的例子。近年来，人们的注意力已经转移到百岁后代（CO）作为一个模型的人遗传健康老龄化和长寿。在这里，我们描述了一个新的记忆B细胞群体的初步特征，定义为CD 19（+）CD 38（-）CD 24（-），我们发现在老年人中的频率更高，但在CO中的频率低于健康年龄匹配的随机对照。此外，我们发现这些细胞上Toll样受体相关分子RP 105（CD 180）的表达降低。CD 180下调可能是免疫衰老的标志。此外，我们发现这些CD 19（+）CD 38（-）CD 24（-）B细胞产生TNF，并假设它们在老年人中观察到的扩增可能有助于增加炎症状态，有时称为“炎症老化”。".
The B cell arm of adaptive immunity undergoes significant modifications with age. Elderly people are characterized by impaired B cell responses reflected in a reduced ability to effectively respond against viruses and bacteria. Alterations of immunity with advancing age (immunosenescence) have been widely studied in centenarians who are considered a good example of successful aging. In recent years, attention has shifted to centenarian offspring (CO) as a model of people genetically advantaged for healthy aging and longevity. Here, we describe the preliminary characterization of a proposed new population of memory B cells, defined as CD19(+)CD38(-)CD24(-), which we find at higher frequencies in the elderly but less so in CO than healthy age-matched random controls. In addition, we found a decreased expression of RP105 (CD180), a toll-like receptor-associated molecule, on these cells. CD180 downregulation may potentially be a marker of immunosenescence. Moreover, we show that these CD19(+)CD38(-)CD24(-) B cells produce TNF and hypothesize that their observed expansion in the elderly might contribute to the increased inflammatory status sometimes designated "inflamm-aging.".