ELECTROPHORESIS OF DUPLEX DEOXYRIBONUCLEIC-ACID IN MULTIPLE-CONCENTRATION AGAROSE GELS - FRACTIONATION OF MOLECULES WITH MOLECULAR-WEIGHTS BETWEEN 2 X 106 AND 110 X 106
ELECTROPHORESIS OF DUPLEX DEOXYRIBONUCLEIC-ACID IN MULTIPLE-CONCENTRATION AGAROSE GELS - FRACTIONATION OF MOLECULES WITH MOLECULAR-WEIGHTS BETWEEN 2 X 106 AND 110 X 106
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DOI：
10.1021/bi00554a026
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
SERWER, P
中科院分区：
文献类型：
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作者：
SERWER, P
通过使用多浓度琼脂糖凝胶，对于分子量在1.88 × 1.88之间的分子，测量在琼脂糖凝胶电泳期间线性双链体[T4、T5和T7噬菌体] DNA迁移的距离，作为琼脂糖浓度（A）和DNA分子量（MW）的函数。106和110 ×106.对于分子量在5.47 × 10 - 15之间的DNA分子，发现了获得对数分子量作为迁移距离（D）的函数的线性图的条件。106和110 ×106;描述了用于分级分离这些分子的改进条件。MW为26.5 × 26.5的DNA的D比分子量为77 × 106的DNA的D。106作为A和电场（E）的函数绘制。通过使用0.075%至0.70%之间的A值和常数E，该图具有单个最大值。上述比值的最大值是E的减函数，在此最大值处的A也是E的减函数。
By use of multiple-concentration agarose gels, the distance migrated by linear, duplex [T4, T5 and T7 bacteriophages] DNA during agarose gel electrophoresis was measured as a function of agarose concentration (A) and MW of the DNA (MW) for molecules with an MW between 1.88 .times. 106 and 110 .times. 106. Conditions for obtaining a linear plot of log MW as a function of distance migrated (D) were found for DNA molecules with an MW between 5.47 .times. 106 and 110 .times. 106; improved conditions for fractionating such molecules are described. The ratio of the D of a DNA with an MW of 26.5 .times. 106 to the D of a DNA with an MW of 77 .times. 106 was plotted as a function of A and electrical field (E). By use of A values between 0.075 and 0.70% and a constant E, this plot had a single maximum. The maximal value of the above ratio was a decreasing function of E, and the A at this maximal value was also a decreasing function of E.