Preliminary Study of Time to Recovery of Rat Sciatic Nerve from High Frequency Alternating Current Nerve Block.
Preliminary Study of Time to Recovery of Rat Sciatic Nerve from High Frequency Alternating Current Nerve Block.
复制标题
高频交流神经阻滞大鼠坐骨神经恢复时间的初步研究。
DOI：
10.1109/embc.2018.8512832
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Rapeaux A
中科院分区：
文献类型：
--
作者：
Rapeaux A
高频交流神经阻滞在神经调节治疗方面具有很大的潜力。然而，还没有对信号关闭后神经从阻塞中恢复所需的时间进行精确测量。本研究旨在研究大鼠坐骨神经在不同幅度和频率下阻断30秒后恢复的时间。在体内进行实验，以量化恢复时间和恢复完整性，从块结束0.7S。用幅度为2~9 mA、频率为10~50 kHz的交变方波信号阻断坐骨神经。为了确定恢复动力学，在阻断刺激停止后，以100赫兹刺激神经。在试验期间，测量了腓肠肌内侧肌和胫前肌的肌电信号，作为神经功能的指标。这使得能够以10ms的分辨率测量神经恢复。这一分辨率比文献中以前对神经恢复的测量要大得多。神经恢复到稳定活动状态的时间从20到430毫秒不等，最终在0.7秒时的相对恢复活动大约跨度为0.2到1。较高的阻塞信号幅度增加了恢复时间，降低了恢复完整性。这些结果表明，阻断信号的性质会影响神经恢复动力学，这可能有助于改进神经调节疗法，并允许使用不同的阻断信号参数对研究结果进行更准确的比较。
High-Frequency alternating current nerve block has great potential for neuromodulation-based therapies. However, no precise measurements have been made of the time needed for nerves to recover from block once the signal has been turned off. This study aims to characterise time to recovery of the rat sciatic nerve after 30 seconds of block at varying amplitudes and frequencies. Experiments were carried out in-vivo to quantify recovery times and recovery completeness within 0.7 s from the end of block. The sciatic nerve was blocked with an alternating square wave signal of amplitude and frequency ranging from 2 to 9mA and 10 to 50 kHz respectively. To determine the recovery dynamics the nerve was stimulated at 100 Hz after cessation of the blocking stimulus. Electromyogram signals were measured from the gastrocnemius medialis and tibialis anterior muscles during trials as indicators of nerve function. This allowed for nerve recovery to be measured with a resolution of 10 ms. This resolution is much greater than previous measurements of nerve recovery in the literature. Times for the nerve to recover to a steady state of activity ranged from 20 to 430 milliseconds and final relative recovery activity at 0.7 seconds spanned 0.2 to 1 approximately. Higher blocking signal amplitudes increased recovery time and decreased recovery completeness. These results suggest that blocking signal properties affect nerve recovery dynamics, which could help improve neuromodulation therapies and allow more precise comparison of results across studies using different blocking signal parameters.