Principles and Methods of Immunohistochemistry
Principles and Methods of Immunohistochemistry
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DOI：
10.1007/978-1-60761-849-2_5
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Ramos-Vara, Jose A.
中科院分区：
文献类型：
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作者：
Ramos-Vara, Jose A.
免疫组织化学技术通过免疫学和化学反应检测组织切片中的抗原。该技术具有高度的灵敏度和特异性，可以检测多种动物物种中的各种抗原。本章回顾了常见的免疫组化方法用于正常和病理组织的表征和所用的试剂。包括预处理，如内源性活性的封闭步骤和抗原修复。强调了福尔马林固定、石蜡包埋组织的标准程序以及新抗体的方法标准化和故障排除。
Immunohistochemical techniques detect antigens in tissue sections by means of immunological and chemical reactions. This technique is highly sensitive and specific and can detect a wide variety of antigens in multiple animal species. This chapter reviews common immunohistochemical methods used in the characterization of normal and pathologic tissue and the reagents used. Pretreatments such as blocking steps for endogenous activities and antigen retrieval are included. Standard procedures on formalin-fixed, paraffin-embedded tissues as well as method standardization for new antibodies and troubleshooting are emphasized.