Increased Expression of TGF-β1 in Correlation with Liver Fibrosis during Echinococcus granulosus Infection in Mice.
Increased Expression of TGF-β1 in Correlation with Liver Fibrosis during Echinococcus granulosus Infection in Mice.
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DOI：
10.3347/kjp.2016.54.4.519
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发表时间：
2016-08
期刊：
影响因子：
--
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Liu Y;Abudounnasier G;Zhang T;Liu X;Wang Q;Yan Y;Ding J;Wen H;Yimiti D;Ma X
为探讨转化生长因子-β1（TGF-β1）在细粒棘球蚴感染肝纤维化中的作用，将96只BALB/c小鼠随机分为2组，实验组腹腔注射棘球蚴悬液，对照组给予生理盐水。分别于感染后第2、8、30、90、180、270 d采集肝脏和血液标本，采用实时荧光定量RT-PCR和ELISA法检测TGF-β1 mRNA和蛋白的表达。我们还用苏木精-伊红（HE）和Masson染色的肝脏切片评价了感染期间肝脏的病理变化。H-E染色的感染肝切片的病理学分析显示肝细胞水肿、胆管增生和肝结构损伤，如感染肝的不清晰可见的小叶结构、肝细胞空泡变性和感染后期淋巴细胞浸润所证明的。对照组小鼠的肝组织切片保持正常。Masson染色显示感染末期肝纤维化加重。感染早期TGF-β1水平无明显变化，感染中晚期TGF-β1水平明显升高（P<0.05）。RT-PCR结果显示，感染早期TGF-β1 mRNA表达水平较低，与对照组相当，感染后30 d显著升高，并持续至感染后270 d（P<0.05）。本研究结果提示，E.颗粒体感染可能在E.颗粒体感染
To investigate the potential role of transforming growth factor (TGF)-β1 in liver fibrosis during Echinococcus granulosus infection, 96 BALB/c mice were randomly divided into 2 groups, experimental group infected by intraperitoneal injection with a metacestode suspension and control group given sterile physiological saline. The liver and blood samples were collected at days 2, 8, 30, 90, 180, and 270 post infection (PI), and the expression of TGF-β1 mRNA and protein was determined by real-time quantitative RT-PCR and ELISA, respectively. We also evaluated the pathological changes in the liver during the infection using hematoxylin and eosin (H-E) and Masson staining of the liver sections. Pathological analysis of H-E stained infected liver sections revealed liver cell edema, bile duct proliferation, and structural damages of the liver as evidenced by not clearly visible lobular architecture of the infected liver, degeneration of liver cell vacuoles, and infiltration of lymphocytes at late stages of infection. The liver tissue sections from control mice remained normal. Masson staining showed worsening of liver fibrosis at the end stages of the infection. The levels of TGF-β1 did not show significant changes at the early stages of infection, but there were significant increases in the levels of TGF-β1 at the middle and late stages of infection (P<0.05). RT-PCR results showed that, when compared with the control group, TGF-β1 mRNA was low and comparable with that in control mice at the early stages of infection, and that it was significantly increased at day 30 PI and remained at high levels until day 270 PI (P<0.05). The results of this study suggested that increased expression of TGF-β1 during E. granulosus infection may play a significant role in liver fibrosis associated with E. granulosus infection.
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