Identification of trans-acting genes necessary for centromere function in Drosophila melanogaster using centromere-defective minichromosomes.
Identification of trans-acting genes necessary for centromere function in Drosophila melanogaster using centromere-defective minichromosomes.
复制标题
使用着丝粒缺陷微型染色体鉴定果蝇着丝粒功能所需的反式作用基因。
DOI：
10.1093/genetics/145.3.737
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Karpen,GH
中科院分区：
文献类型：
--
作者：
Cook,KR;Murphy,TD;Nguyen,TC;Karpen,GH
果蝇小染色体dp1187的缺失被用来研究反式作用基因与着丝粒的遗传相互作用。已知在染色体遗传中起作用的几个基因的突变被证明对具有部分着丝粒缺陷的小染色体的稳定性具有显性影响。thencdandklp3akinesin样蛋白基因的杂合突变强烈地降低了遗传上确定的着丝粒缺失部分的小染色体的传递，但对具有完整着丝粒的小染色体的传递几乎没有影响。使用这种方法，ncdand klp3a被证明只需要染色体的着丝粒区域来完成它们在染色体分离中的作用。增加的基因剂量也会影响小染色体的传播，并被用来证明，除了与先前证明的中心体外区域相互作用外，thenodkinesin样蛋白基因还与着丝粒相互作用。本研究的结果强烈表明，突变和着丝粒缺陷小染色体之间的显性遗传相互作用可以有效地用于鉴定着丝粒功能所需的新基因。
Deletions in the Drosophila minichromosomeDp1187were used to investigate the genetic interactions oftrans-acting genes with the centromere. Mutations in several genes known to have a role in chromosome inheritance were shown to have dominant effects on the stability of minichromosomes with partially defective centromeres. Heterozygous mutations in thencdandklp3Akinesin-like protein genes strongly reduced the transmission of minichromosomes missing portions of the genetically defined centromere, but had little effect on the transmission of minichromosomes with intact centromeres. Using this approach,ncdandklp3Awere shown to require only the centromeric region of the chromosome for their roles in chromosome segregation. Increased gene dosage also affected minichromosome transmission and was used to demonstrate that thenodkinesin-like protein gene interacts genetically with the centromere, in addition to interacting with extracentromeric regions as demonstrated previously. The results presented in this study strongly suggest that dominant genetic interactions between mutations and centromere-defective minichromosomes could be used effectively to identify novel genes necessary for centromere function.
影响因子：
2.5
作者：
A. Abrams;M. Clark;F. L. Crane;Y. Galante;M. A. Harmey;A. Linnane;S. Marzuki;Scott Mathews;R. E. Mccarty
通讯作者：
R. E. Mccarty