CRF2-4: Isolation of cDNA clones encoding the human and mouse proteins
CRF2-4: Isolation of cDNA clones encoding the human and mouse proteins
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DOI：
10.1016/s0378-1119(96)00690-7
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发表时间：
1997-02-20
期刊：
影响因子：
3.5
通讯作者：
Pennica, D
中科院分区：
文献类型：
--
作者：
Gibbs, VC;Pennica, D
为了寻找编码人干扰素受体分子的基因，我们将外显子捕获技术应用于人21号染色体酵母人工染色体（YACs）。我们发现了一个编码人CRF2-4的全长cDNA，该cDNA在人体组织中广泛表达。以人CRF2-4 cDNA为探针，筛选小鼠巨噬细胞cDNA文库。小鼠CRF2-4 cDNA编码349个氨基酸（aa）的多肽，在aa水平上与人类相同69%。小鼠CRF2-4预计是干扰素受体II类细胞因子受体家族的另一个成员。
To search for genes which encode human interferon receptor molecules, we applied the technique of exon trapping to human chromosome 21 yeast artificial chromosomes (YACs). A full length cDNA encoding human CRF2-4 was identified which is shown here to be widely expressed in human tissues. The human CRF2-4 cDNA was used as a probe to screen a mouse macrophage cDNA library. A mouse CRF2-4 cDNA was isolated which encodes a 349-amino-acid (aa) polypeptide that is 69% identical at the aa level with its human counterpart. Mouse CRF2-4 is predicted to be another member of the interferon receptor-class II cytokine receptor family.