A systematic survey of lipids across mouse tissues
A systematic survey of lipids across mouse tissues
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DOI：
10.1152/ajpendo.00371.2013
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发表时间：
2014-04-01
影响因子：
5.1
通讯作者：
Nilsson, Roland
中科院分区：
文献类型：
--
作者：
Jain, Mohit;Ngoy, Soeun;Nilsson, Roland
脂质是正常生理所必需的大分子的多样性集合，但许多单个脂质种类的组织分布和功能仍不清楚。在这里，我们报告了18个小鼠组织中脂质丰度的质谱调查，检测到类似于1，000个质谱特征，其中我们从甘油脂，甘油磷脂，溶血磷脂，酰基肉毒碱，鞘脂和胆固醇酯类中识别出179种脂质。我们的数据揭示了脂质的组织特异性组织，并可用于生成可检验的假设。例如，我们的数据表明，与人类未来糖尿病正相关和负相关的循环甘油三酯分别在小鼠脂肪组织和肝脏中富集，从而提出了关于这些糖尿病相关脂质的组织来源的假设。我们还将我们的组织脂质数据与基因表达谱相结合，以预测一些脂质代谢酶的底物，突出胆碱磷酸转移酶和甾醇O-酰基转移酶。最后，我们确定了几种组织特异性脂质不存在于血浆中，在正常条件下，可能是感兴趣的组织损伤的生物标志物，我们表明，这些脂质中的两个释放到血液中缺血性脑损伤小鼠。这一资源补充了现有的组织基因表达纲要，并可能是有用的综合生理学和脂质生物学。
Lipids are a diverse collection of macromolecules essential for normal physiology, but the tissue distribution and function for many individual lipid species remain unclear. Here, we report a mass spectrometry survey of lipid abundance across 18 mouse tissues, detecting similar to 1,000 mass spectrometry features, of which we identify 179 lipids from the glycerolipids, glycerophospholipids, lysophospholipids, acylcarnitines, sphingolipids, and cholesteryl ester classes. Our data reveal tissue-specific organization of lipids and can be used to generate testable hypotheses. For example, our data indicate that circulating triglycerides positively and negatively associated with future diabetes in humans are enriched in mouse adipose tissue and liver, respectively, raising hypotheses regarding the tissue origins of these diabetes-associated lipids. We also integrate our tissue lipid data with gene expression profiles to predict a number of substrates of lipid-metabolizing enzymes, highlighting choline phosphotransferases and sterol O-acyltransferases. Finally, we identify several tissue-specific lipids not present in plasma under normal conditions that may be of interest as biomarkers of tissue injury, and we show that two of these lipids are released into blood following ischemic brain injury in mice. This resource complements existing compendia of tissue gene expression and may be useful for integrative physiology and lipid biology.