Facile synthesis of orange fluorescence carbon dots with excitation independent emission for pH sensing and cellular imaging
Facile synthesis of orange fluorescence carbon dots with excitation independent emission for pH sensing and cellular imaging
复制标题
轻松合成具有激发独立发射的橙色荧光碳点，用于 pH 传感和细胞成像
DOI：
10.1016/j.aca.2018.08.044
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发表时间：
2018-12-26
影响因子：
6.2
通讯作者：
Dong, Chuan
中科院分区：
文献类型：
--
作者：
Jiao, Yuan;Gong, Xiaojuan;Dong, Chuan
High-efficient orange fluorescence nitrogen-doped carbon dots (N-CDs) were facilely prepared from P-phenylenediamine as a precursor via hydrothermal method. The as-prepared N-CDs with an average diameter of 3.65 nm displayed excitation-independent emission at 590 nm. The N-CDs demonstrated a remarkable fluorescence enhancement behavior with the increase of pH. A sigmoidal curve was well fitted using BiDoseResp equation with pK(a1) 3.57 and pK(a2) 6.01, which can be ascribed to the unique surface properties of N-CDs. Two-segment linear ranges of 2.6-4.6 and 5.0-6.8 broaden the response range to pH of the orange-emission N-CDs to some extent. The confocal fluorescent microscopic images of SMMC7721 cells were performed successfully, which demonstrating that N-CDs possess exceptional cell membrane permeability and can implement as biosensing platform to monitor pH fluctuations in living cells. (C) 2018 Elsevier B.V. All rights reserved.
