New selective medium for isolation of Burkholderia glumae from rice seeds
New selective medium for isolation of Burkholderia glumae from rice seeds
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DOI：
10.1007/pl00012951
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发表时间：
2000-08-01
影响因子：
1.2
通讯作者：
Kusakari, Shin'ichi
中科院分区：
文献类型：
--
作者：
Kawaradani, Mitsuo;Okada, Kiyotsugu;Kusakari, Shin'ichi
研制了一种新的选择性培养基，与目前使用的Tsushima的S-PG培养基相比，该培养基组成简单，选择性更高。该选择性培养基，命名为CCNT，在1000 ml蒸馏水中含有2 g酵母提取物、1 g多肽、4 g肌醇、10 mg溴化十六烷基三甲铵、10 mg氯霉素、1 mg新生霉素、100 mg百菌清和18 g琼脂，并将pH调节至4.8。B。在41 ℃培养2 - 4天后，在CCNT培养基上，颖壳菌产生一个淡黄色的白色菌落，菌落内有可扩散的黄色色素，可与其它细菌种区分开来。在CCNT培养基上，B.在水稻种子中，在S-PG培养基上以类似的速率检测到颖壳菌，而以低得多的速率检测到其他微生物。
A new selective medium for Burkholderia glumae was developed, which has a simpler composition and greater selectivity compared to Tsushima's S-PG medium currently used. This selective medium, designated CCNT, contains 2 g of yeast extract, 1 g of polypepton, 4 g of inositol, 10 mg of cetrimide, 10 mg of chloramphenicol, 1 mg of novobiocin, 100 mg of chlorotharonil and 18 g of agar in 1000 ml of distilled water, and is adjusted to pH 4.8. B. glumae produced a yellowish white colony with a diffusible yellow pigment on CCNT medium, which was distinguishable from other bacterial species when incubated at 41degreeC for 2 to 4 days. On CCNT medium, B. glumae was detected at a rate similar to that on S-PG medium in rice seeds, while other microorganisms were detected at a much lower rate.