Proton resonance frequency chemical shift thermometry: experimental design and validation toward high-resolution noninvasive temperature monitoring and in vivo experience in a nonhuman primate model of acute ischemic stroke.
Proton resonance frequency chemical shift thermometry: experimental design and validation toward high-resolution noninvasive temperature monitoring and in vivo experience in a nonhuman primate model of acute ischemic stroke.
复制标题
DOI：
10.3174/ajnr.a4241
复制
发表时间：
2015-06
期刊：
影响因子：
--
通讯作者：
Oshinski JN
中科院分区：
文献类型：
--
作者：
Dehkharghani S;Mao H;Howell L;Zhang X;Pate KS;Magrath PR;Tong F;Wei L;Qiu D;Fleischer C;Oshinski JN
非侵入性生物温度监测的应用在生物医学中是广泛的，并且在脑温度调节的背景下是特别感兴趣的，其中传统上昂贵且侵入性的监测方案限制了它们在许多环境中的适用性。因此，脑热调节仍然存在争议，推动了非侵入性方法的发展，如温度敏感的NMR现象。这项工作的目的是比较实用的竞争方法，以MR测温（MRT）采用质子共振频率化学位移。三种方法进行了测试，假设一个快速和准确的方法来化学位移测温的可行性，在幻影研究在3.0特斯拉。一个传统的，成对的方法（DIFF-1），加速单扫描方法（DIFF-2），和一个新的，进一步加速的策略（DIFF-3）进行了测试。在实时光纤温度监测期间调制体模温度，同时从水质子化学位移（~0.01 ppm/°C）的温度敏感变化中获得MRT。随后在一系列生理和缺血条件下的体内非人灵长类动物实验中进行MRT，测试其重现性和总体性能。对于所有三种方法，化学位移温度测定法均显示与体模温度的良好一致性（DIFF-1线性回归R2=0.994，p<0.001，采集时间4 min 40 s; DIFF-2 R2=0.996，p<0.001，采集时间4 min; DIFF-3 R2=0.998，p<0.001，采集时间40 s）。这些研究结果证实了三种竞争方法MRT的性能具有可比性，并在灵长类动物中风模型的生理和缺血条件下提出了体内应用。
Applications for non-invasive biological temperature monitoring are widespread in biomedicine, and of particular interest in the context of brain temperature regulation, where traditionally costly and invasive monitoring schemes limit their applicability in many settings. Brain thermal regulation therefore remains controversial, motivating the development of non-invasive approaches such as temperature-sensitive NMR phenomena. The purpose of this work was to compare the utility of competing approaches to MR thermometry (MRT) employing proton resonance frequency chemical shift. Three methodologies were tested, hypothesizing the feasibility of a fast and accurate approach to chemical shift thermometry, in a phantom study at 3.0 Tesla. A conventional, paired approach (DIFF-1), an accelerated single-scan approach (DIFF-2), and a new, further accelerated strategy (DIFF-3) were tested. Phantom temperatures were modulated during real-time fiber optic temperature monitoring, with MRT derived simultaneously from temperature-sensitive changes in the water proton chemical shift (~0.01 ppm/°C). MRT was subsequently performed in a series of in vivo non-human primate experiments under physiologic and ischemic conditions testing its reproducibility and overall performance. Chemical shift thermometry demonstrated excellent agreement with phantom temperatures for all three approaches (DIFF-1 linear regression R2=0.994, p<0.001, acquisition time 4 min 40 s; DIFF-2 R2=0.996, p<0.001, acquisition time 4 min; DIFF-3 R2=0.998, p<0.001, acquisition time 40 s). These findings confirm the comparability in performance of three competing approaches MRT, and present in vivo applications under physiologic and ischemic conditions in a primate stroke model.