LOCALIZATION OF GENE AVG FOR ANTIVIRAL EXPRESSION OF IMMUNE AND CLASSICAL INTERFERON TO DISTAL PORTION OF LONG ARM OF CHROMOSOME-21
LOCALIZATION OF GENE AVG FOR ANTIVIRAL EXPRESSION OF IMMUNE AND CLASSICAL INTERFERON TO DISTAL PORTION OF LONG ARM OF CHROMOSOME-21
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DOI：
10.1093/infdis/133.supplement_2.a56
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发表时间：
1976-01-01
影响因子：
6.4
通讯作者：
EPSTEIN, CJ
中科院分区：
文献类型：
--
作者：
EPSTEIN, LB;EPSTEIN, CJ
正常的成纤维细胞和成纤维细胞三体和单体的染色体21外源性给药的病毒诱导（经典）和植物血凝素诱导（免疫）的人干扰素的反应进行了测定。从用仙台病毒处理的白细胞和用纽卡斯尔病病毒处理的新生儿包皮成纤维细胞中获得病毒诱导的干扰素。与经典和免疫干扰素，三体细胞系的平均响应是正常细胞的三倍，而单体线的一半或更少的正常细胞。此外，仅21号染色体长臂远侧一半的三体系（q21 → qter）也证明了对病毒和植物血凝素诱导的干扰素的敏感性增加，这一事实表明负责干扰素抗病毒作用的基因AVG位于21号染色体的这一部分。对两类干扰素（病毒诱导的和植物血凝素诱导的）的不同敏感程度的个体细胞系的反应强烈相关（r= 0.79）。因此，可以得出结论，尽管它们的物理和抗原差异，经典和免疫干扰素的抗病毒表达最终介导的相同的遗传位点，AVG。
The responses of normal fibroblasts and of fibroblasts trisomic and monosomic for chromosome 21 to exogenously administered virus-induced (classical) and phytohemagglutinin-induced (immune) human interferon were determined. The virus-induced interferon was obtained from leukocytes treated with Sendai virus and from neonatal foreskin fibroblasts treated with Newcastle disease virus. With both classical and immune interferons, the mean response of the trisomic cell lines was three times that of the normal cells, whereas that of the monosomic lines was half or less that of the normal cells. Furthermore, a line trisomic for only the distal half of the long arm of chromosome 21 (q21 → qter) also demonstrated increased sensitivity to virus- and phytohemagglutinin-induced interferons, a fact that indicated that the gene responsible for the antiviral effect of interferon,AVG, is located on this part of chromosome 21. Responses to the two categories of interferon (virus-induced and phytohemagglutinin-induced) of individual cell lines of different degrees of sensitivity were strongly correlated (r= 0.79). It is concluded, therefore, that despite their physical and antigenic differences, the antiviral expressions of both classical and immune interferons are ultimately mediated by the same genetic locus,AVG.