ELECTRON-MICROSCOPY OF DEFINED LENGTHS OF CHROMATIN

ELECTRON-MICROSCOPY OF DEFINED LENGTHS OF CHROMATIN
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DOI:
10.1073/pnas.72.9.3320
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发表时间:
1975-01-01
影响因子:
11.1
通讯作者:
KORNBERG, RD
KORNBERG, RD
中科院分区:
综合性期刊1区
文献类型:
--
作者:
FINCH, JT;NOLL, M;KORNBERG, RD

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确定长度的染色质是用微球菌核酸酶短暂消化和蔗糖梯度分离制备的。含有200个碱基对重复单位的长度在电子显微镜下表现为相同数量的100个A珠。一段长度内珠粒之间的距离很小,通常小于20 a左右。
Defined lengths of chromatin were prepared by brief digestion with micrococcal nuclease and fractionation in a sucrose gradient. A length containing a given number of 200 base pair repeating units appeared as the same number of 100 A beads in the electron microscope. The distance between beads within a length was small, usually less than about 20 A.