Qualitative Analysis of Mouse Specific-Locus Mutations: Information on Genetic Organization, Gene Expression, and the Chromosomal Nature of Induced Lesions
Qualitative Analysis of Mouse Specific-Locus Mutations: Information on Genetic Organization, Gene Expression, and the Chromosomal Nature of Induced Lesions
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小鼠特异性位点突变的定性分析：有关遗传组织、基因表达和诱发病变的染色体性质的信息
DOI：
10.1007/978-1-4613-3739-3_15
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发表时间：
1983
影响因子：
1.5
通讯作者：
L. B. Russell
中科院分区：
文献类型：
--
作者：
L. B. Russell
在特定位点实验中记录的突变可以有很多用途。当然，它们的主要功能是检测和测量一种试剂[1]的致突变性。由于标记位点上突变的明确表型，这一主要功能得以迅速而准确地实现。但是，如果资源允许建立种群并随后对突变进行遗传分析，所获得的资料可以对其他几个知识领域作出重大贡献。这些信息可以阐明由特定试剂引起的突变的性质，以及某些类型的遗传病变在生物体水平上表达的方式。这些领域的信息需要相互结合起来进行风险评估。第三，这些发现揭示了哺乳动物染色体的遗传组织。最后，它们为进一步研究基础遗传学和诱变提供了工具。本文总结了橡树岭辐射实验中引起的突变对这些领域的贡献。
Mutations scored in specific-locus experiments can serve many purposes. Their primary function, of course, is to detect and measure mutagenicity of an agent [1]. This primary function is fulfilled rapidly and accurately, thanks to the well-defined phenotypes of mutations at the marked loci. However, when resources permit the setting up of stocks and the subsequent genetic analysis of the mutations, the information obtained can make major contributions to several other areas of knowledge. Such information can shed light on the nature of mutations induced by specific agents, and on the manner in which certain types of genetic lesions are expressed on the organismic level. These areas of information are needed, in combination with each other, for an assessment of risk. Thirdly, the findings shed light on the genetic organization in mammalian chromosomes. Finally, they serve to identify tools for further investigations in basic genetics and mutagenesis. This paper summarizes contributions to these areas from findings made on mutations induced in radiation experiments at Oak Ridge.