Microarray and qPCR Analyses of Wallerian Degeneration in Rat Sciatic Nerves.
Microarray and qPCR Analyses of Wallerian Degeneration in Rat Sciatic Nerves.
复制标题
大鼠坐骨神经华勒变性的微阵列和 qPCR 分析
DOI：
10.3389/fncel.2017.00022
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发表时间：
2017
影响因子：
5.3
通讯作者：
Gu X
中科院分区：
文献类型：
--
作者：
Yi S;Tang X;Yu J;Liu J;Ding F;Gu X
沃勒变性在哺乳动物外周神经损伤后立即发生。为了更好地理解沃勒变性过程中发生的分子事件，使用坐骨神经横断的大鼠模型来评估神经损伤（PNI）后0.5、1、6、12、24小时、4天、1、2、3和4周的差异表达基因。层次聚类，欧氏距离矩阵，主成分分析（PCA）共同建议三个不同的阶段内受伤后4周的时间。基因本体（GO）分析表明，I期（0-6 h PNI）、II期（6-24 h PNI）和III期（4天至4周）分别与对损伤的急性反应、沃勒变性的预形成和沃勒变性的完全执行相关。分别使用京都基因和基因组富集（KEGG）途径分析和免疫途径分析（IPA）进一步鉴定和整合负责调节沃勒变性的关键信号传导途径和转录因子网络。我们的研究结果可能有助于阐明与哺乳动物外周神经轴突创伤性损伤后发生的沃勒变性相关的基因调控的一些分子机制。
Wallerian degeneration occurs immediately following injury to mammal peripheral nerves. To better understand the molecular events occurring during Wallerian degeneration, a rat model of sciatic nerve transection was used to assess differentially expressed genes at 0.5, 1, 6, 12, 24 h, 4 days, 1, 2, 3, and 4 weeks post nerve injury (PNI). Hierarchical clustering, Euclidean distance matrix, and principal component analysis (PCA) collectively suggested three distinct phases within the post-injury period of 4 weeks. Gene ontology (GO) analysis suggested that phase I (0–6 h PNI), phase II (6–24 h PNI), and phase III (4 days to 4 weeks) were associated with acute response to injury, preformation of Wallerian degeneration, and complete execution of Wallerian degeneration, respectively. Critical signaling pathways and transcriptional factor networks responsible for the regulation of Wallerian degeneration were further identified and integrated using Kyoto Enrichment of Genes and Genomes (KEGG) pathway analysis and Ingenuity Pathway Analysis (IPA), respectively. Our results may help to elucidate some molecular mechanisms of gene regulation associated with Wallerian degeneration that occurs after traumatic injury to peripheral nerve axons in mammals.