Regulated secretory proteins in the exocrine pancreas aggregate under conditions that mimic the trans-Golgi network.
Regulated secretory proteins in the exocrine pancreas aggregate under conditions that mimic the trans-Golgi network.
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外分泌胰腺中的调节分泌蛋白在模拟跨高尔基体网络的条件下聚集。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
G. Scheele
中科院分区：
文献类型：
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作者：
S. Freedman;G. Scheele
15胰腺分泌蛋白，包括7个丝氨酸内蛋白酶（胰蛋白酶原，胰凝乳蛋白酶原和弹性蛋白酶原的同工酶形式），4个金属外蛋白酶（羧肽酶原A和羧肽酶原B的同工酶形式），淀粉酶，脂肪酶，和两种形式的羧基酯脂肪酶的条件下，观察到聚集酸性pH值（5.5）和钙，模仿trans-Golgi网络。随后的pH值的中和导致蛋白质聚集体的破坏和胰腺（原）酶的溶解。在没有分泌颗粒膜的情况下，颗粒内容物在温和酸化条件下显示可逆的pH依赖性聚集的“内在”性质。
Fifteen pancreatic secretory proteins, including seven serine-endoproteinases (isoenzyme forms of trypsinogen, chymotrypsinogen and proelastase), four metallo-exoproteinases (isoenzymic forms of procarboxypeptidase A and procarboxypeptidase B), amylase, lipase, and two forms of carboxyl ester lipase were observed to aggregate under conditions of acidic pH (5.5) and calcium that mimic the trans-Golgi network. Subsequent neutralization of the pH resulted in disruption of protein aggregates and solubilization of pancreatic (pro)enzymes. In the absence of secretory granule membranes, granule contents display an "intrinsic" property for reversible, pH-dependent aggregation under conditions of mild acidification.