Variation in carbohydrates and screening of related differential proteins during the seed germination of Magnolia sieboldii K. Koch
Variation in carbohydrates and screening of related differential proteins during the seed germination of Magnolia sieboldii K. Koch
复制标题
天女木兰种子萌发过程碳水化合物变化及相关差异蛋白筛选
DOI：
10.1007/s00468-016-1456-8
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发表时间：
2017-02-01
影响因子：
2.3
通讯作者：
Sun, Xiao-mei
中科院分区：
文献类型：
--
作者：
Mei, Mei;Lu, Xiu-jun;Sun, Xiao-mei
关键信息本论文主要利用蛋白质组学技术对天女木兰种子萌发过程中的碳水化合物代谢进行研究。结果表明，糖酵解是天女木兰最重要的代谢途径。科赫公司被选为我们的研究对象。M.首先将天女兰种子在清水中浸泡72小时，然后在波动温度下以种子与砂的比例为1：3在湿砂中分层。通过种子胚发育、种子解剖结构、种子层积过程中碳水化合物的变化和差异蛋白质积累分析，研究了种子层积过程中碳水化合物代谢与种子萌发的关系，以及代谢途径中相关蛋白质的定位。根据这些结果，我们可以将种子休眠和萌发分为三个阶段：胚长不变阶段（I期）、缓慢伸长阶段（II期）和快速伸长阶段（III期）。双向电泳结果表明，在4个种子蛋白样品中，分别对应于层积过程中的4个关键阶段，获得了67个差异蛋白。草蛉种子（层积0、45、90和110天）。KEGG代谢途径分析表明，67个差异蛋白中，7个与碳代谢相关，分别位于10条代谢途径中，4个位于糖酵解/异源生成途径中，在种子萌发早期表达上调。因此，在这一阶段，种子萌发所需的能量是由糖酵解/异源生殖提供的。
Key messageThis paper focuses on carbohydrate metabolism ofMagnolia sieboldiiduring seed germination by proteomics. 10 differentially accumulated proteins were found. Glycolysis is the most important metabolic pathways.AbstractMagnolia sieboldii K. Koch was selected as our research object. M. sieboldii seeds were initially soaked in clean water for 72 h and then stratified in wet sand with a seed-to-sand ratio of 1:3 at a fluctuating temperature. The relationship between carbohydrate metabolism and seed germination, and the localization of related proteins in metabolic pathways were investigated through seed embryo development, seed anatomical structure, carbohydrate change, and differentially accumulated protein analysis during seed stratification. On the basis of the results, we can divide seed dormancy and germination into three stages: unchanging embryo length (phase I), slow stretching phase (phase II), and rapid elongation phase (phase III). Two-dimensional electrophoresis revealed that 67 differentially accumulated proteins were obtained in four seed protein samples corresponding to the four key stages during the stratification of M. sieboldii seeds (0, 45, 90, and 110 days of stratification). Of the 67 differentially accumulated proteins, 7 were related to carbon metabolism and localized in 10 metabolic pathways and 4 were located in the glycolysis/gluconeogenesis pathway and upregulated in the early stage of seed germination, as indicated by KEGG metabolic pathway analysis. Thus, energy for seed germination was provided by glycolysis/gluconeogenesis in this stage.