Immune response of mice with alveolar echinococcosis to therapy with transfer factor, alone and in combination with albendazole
Immune response of mice with alveolar echinococcosis to therapy with transfer factor, alone and in combination with albendazole
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DOI：
10.1007/s00436-009-1520-z
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发表时间：
2009-10-01
影响因子：
2
通讯作者：
Sevcikova, Zuzana
中科院分区：
文献类型：
--
作者：
Dvoroznakova, Emilia;Porubcova, Jarmila;Sevcikova, Zuzana
观察可透析白细胞提取物（转移因子TF）对感染多房棘球蚴并接受阿苯达唑（ABZ）治疗的小鼠免疫反应的影响。从感染后第 8 周到第 12 周或第 14 周，TF 给药分别增加了受感染小鼠 T 和 B 淋巴细胞的寄生虫抑制增殖反应，其中 TF+ABZ 治疗后的刺激作用最大。接受 TF 或 TF+ABZ 治疗的感染小鼠脾脏中 CD4 T 细胞的存在分别从注射后第 6 周增加到第 12 周或第 14 周。 TF 或 TF+ABZ 治疗后 IFN-γ（Th1 细胞因子）的产生在注射后第 6 周至 12 周显着升高，在此期间，检测到显着抑制的 IL-5 合成（Th2 细胞因子），特别是在 TF+ABZ 治疗后。用TF或TF+ABZ治疗的受感染小鼠的腹膜巨噬细胞中超氧阴离子（O (2) (-) ）的产生在注射后第8周至第18周受到刺激。 TF 的免疫调节作用可减少幼虫包囊的生长，直至注射后第 14 周。与驱虫药 ABZ 具有相当的强度。联合疗法TF+ABZ导致最大程度的寄生虫限制并减少包囊发育直至实验结束。
The effect of dialysable leucocyte extract (transfer factor TF) on immune response of mice infected with Echinococcus multilocularis and treated with albendazole (ABZ) was observed. TF administration increased the parasite-suppressed proliferative response of T and B lymphocytes of infected mice from weeks 8 to 12 or 14 post infection (p.i.), respectively, with the most stimulative effect after TF+ABZ therapy. The CD4 T cell presence in the spleen of infected mice with TF or TF+ABZ therapy was increased from weeks 6 to 12 or 14 p.i., respectively. The production of IFN-gamma (Th1 cytokine) after TF or TF+ABZ therapy was significantly higher from weeks 6 to 12 p.i., and during this time, the significantly inhibited IL-5 synthesis (Th2 cytokine) was detected, particularly after TF+ABZ therapy. The superoxide anion (O (2) (-) ) production in peritoneal macrophages of infected mice treated with TF or TF+ABZ was stimulated from weeks 8 to 18 p.i. The immunomodulative effect of TF reduced the growth of larval cysts till week 14 p.i. with a comparable intensity to the anthelmintic drug ABZ. Combined therapy TF+ABZ resulted in the greatest parasite restriction and reduced the cyst development till the end of the experiment.