Ontogeny of angiotensin II receptors, types 1 and 2, in ovine mesonephros and metanephros
Ontogeny of angiotensin II receptors, types 1 and 2, in ovine mesonephros and metanephros
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DOI：
10.1038/ki.1997.376
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发表时间：
1997-09-01
影响因子：
19.6
通讯作者：
Wintour, EM
中科院分区：
文献类型：
--
作者：
Butkus, A;Albiston, A;Wintour, EM
通过RNA酶保护试验、杂交组织化学和体外放射自显影，证明妊娠40天（足月约150天）的绵羊胎儿中肾和后肾中存在AT（1）和AT（2）受体的mRNA和蛋白。AT（1）mRNA定位于40、75、131天胎龄和2天龄羔羊肾小球的假定系膜细胞，除了广泛存在于皮质和髓质的间质细胞，一旦这些区域形成（60天）。出生后2天，髓质AT（1）分布局限于外髓质的内条。AT（2）mRNA在40 ~ 131天（肾发生活跃期）的中肾外周间质/组织和发育中的肾小球周围的间质组织中表达，但在后肾最外层的生肾间质中不表达。此外，AT（2）mRNA定位于致密斑的上皮细胞在后肾（40至131妊娠天）期间，但不是完成后，肾发生。这些研究表明，血管紧张素II（Ang II）可能有分化的影响，通过AT（1）受体，从非常早期的发展。致密斑中AT（2）表达的独特上皮部位提高了Ang II可能通过该受体在致密斑在其肾小球极的不变定位中发挥作用的可能性。
By RNAse protection assay, hybridization histochemistry, and in vitro autoradiography it was shown that both mRNA and protein for AT(1) and AT(2) receptors were present in ovine fetal meso- and metanephroi at 40 days of gestation (term approximate to 150 days). AT(1) mRNA was localized to presumptive mesangial cells of glomeruli at 40-, 75-, 131-gestational-day-old fetuses and two-day-old lambs, in addition to being widely present in interstitial cells of the cortex and medulla, once these zones formed (60 days). By two days after birth the medullary AT(1) distribution was confined to the inner stripe of the outer medulla. AT(2) mRNA was present in peripheral interstitial/tissue of the mesonephros, and interstitial tissue surrounding developing glomeruli, but not the outermost nephrogenic mesenchyme in the metanephros from 40 to approximate to 131 days (the period of active nephrogenesis). In addition, AT(2) mRNA was localized to epithelial cells of the macula densa in metanephroi (40 to 131 gestional days) during, but not after completion, of nephrogenesis. These studies suggest that angiotensin II (Ang II) could have differentiating effects, via AT(1) receptors, from very early in development. The unique epithelial site of AT(2) expression in the macula densa raises the possibility that Ang II may play a role in the invariant positioning of the macula densa at the pole of its glomerulus, via this receptor.