RAT SCHWANN-CELLS PRODUCE INTERLEUKIN-1
RAT SCHWANN-CELLS PRODUCE INTERLEUKIN-1
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DOI：
10.1016/0165-5728(91)90094-n
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发表时间：
1991-10-01
影响因子：
3.3
通讯作者：
JESSEN, KR
中科院分区：
文献类型：
--
作者：
BERGSTEINSDOTTIR, K;KINGSTON, A;JESSEN, KR
越来越多的证据表明，周围神经的雪旺细胞可能能够作为辅助细胞，通过表达主要组织相容性复合物 (MHC) II 类分子，在 T 细胞介导的免疫反应中与免疫系统相互作用。除了 MHC II 类相关抗原向 T 淋巴细胞呈递之外，共刺激因子白细胞介素-1 (IL-1) 的释放也是辅助细胞激活 T 细胞的重要功能。在本研究中，我们研究了雪旺细胞是否能够产生 IL-1。将新生和成年大鼠雪旺细胞的纯化培养物与各种刺激剂一起孵育。从这些培养物中收集上清液和细胞裂解物并测定IL-1活性。新生和成年大鼠雪旺细胞均产生响应细菌抗原的 IL-1 活性，并且细胞裂解物中的 IL-1 活性通常高于上清液中的 IL-1 活性。当用抗IL-1抗体检查刺激的新生或成年大鼠雪旺细胞时，细胞内观察到强烈的免疫标记，但在细胞表面未检测到IL-1。由于IL-1在免疫反应的启动中发挥重要作用，这些观察结果支持雪旺细胞可能作为抗原呈递细胞发挥作用，从而参与周围神经内的神经免疫反应的观点。
There is increasing evidence that Schwann cells of peripheral nerves may be able to function as accessory cells, interacting with the immune system in T cell-mediated immune responses, by expression of the major histocompatibility complex (MHC) class II molecules. In addition to MHC class II-associated presentation of antigen to T lymphocytes, the release of a co-stimulatory factor, interleukin-1 (IL-1), is an essential function of accessory cells for T cell activation. In this study, we investigated if Schwann cells were able to produce IL-1. Purified cultures of neonatal and adult rat Schwann cells were incubated with various stimulatory agents. Supernatants and cell lysates were collected from these cultures and IL-1 activity was assayed. Both neonatal and adult rat Schwann cells produced IL-1 activity in response to bacterial antigens and the IL-1 activity was often higher in the cell lysate than in the supernatant. When stimulated neonatal or adult rat Schwann cells were examined with antibody against IL-1, strong immunolabelling was seen intracellularly, but no IL-1 was detected on the cell surface. Since IL-1 plays an important role in the initiation of immune responses, these observations support the view that Schwann cells may function as antigen-presenting cells, thereby taking part in neuroimmunological responses within peripheral nerves.