Similar potency ratios of amphetamine optical isomers for inhibition of dopamine uptake by synaptosomes of corpus striatum, olfactory tubercle and prefrontal cortex of the rat.
Similar potency ratios of amphetamine optical isomers for inhibition of dopamine uptake by synaptosomes of corpus striatum, olfactory tubercle and prefrontal cortex of the rat.
复制标题
安非他明旋光异构体抑制大鼠纹状体、嗅结节和前额皮质突触体摄取多巴胺的效价比相似。
DOI：
10.1016/0006-2952(84)90702-0
复制
发表时间：
1984
影响因子：
5.8
通讯作者：
Shore,PA
中科院分区：
文献类型：
--
作者：
Matthews,RT;Shore,PA
Materials and methodsFemale Holtzman albino rats weighing 200-225 g were used in all experiments. Tissue preparation and uptake assays were done by a modified method of Horn et al.[15]. Briefly, rats were killed with chloroform asphyxiation, and brains were quickly removed and chilled in ice-cold saline. Samples of frontal cortex were taken as described by Bockaert et al.[16]. These consisted of a medial-dorsal wedge of cortex rostral to the nucleus accumbens. Samples of corpus striatum and olfactory tubercle were dissected bilaterally. Tissues were weighed and then homogenized with a Teflon pestle in glass tubes in 20 vol. of 250 mM sucrose. The homogenate was centrifuged at 1000 g for 10 min, and the supernatant fraction was used for incubations. Aliquots (100/~ 1) of the supernatant fraction were incubated with 1.9 ml of a modified Krebs-Henseleit buffer (95 mM NaCI, 4.7 mM KCI, 1.25 mM CaC12, 1.2 mM KH2PO4, 1.2 mM MgSO4, 25mM NaHCO3, 4.9 mM pyruvate, 5.4 mM fumarate, 4.9 mM L-glutamate, 11.5 mM glucose, 12.5/tM nialamide, 0.2 mg/ml sodium EDTA, 0.2 mg/ml ascorbic acid) in a shaking water bath (37) under a 5% COJ95% 02 atmosphere. Drugs were added 5 min before [3H]-DA (10-7 M). Incubations were terminated after 5 additional min by rapid filtration through 2.1 cm Whatman GF/C filters and washed twice with 5 ml of ice-cold buffer. Preliminary experiments showed linear accumulation of [3H]-DA for at least 5 min in all three brain area preparations. Filters were extracted with toluene-base scintillation mixture for at least 30 rain followed by estimation of tritium with a Beckman scintillation counter.[3H]-Dopamine (21.5 Ci/mmole) was obtained from New England Nuclear, AMPH isomers from Smith, Kline & French Laboratories, fluoxetine from Lilly Research Lab-
