Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers' diarrhoea
Development of an ERIC sequence typing scheme for Laribacter hongkongensis, an emerging pathogen associated with community-acquired gastroenteritis and travellers' diarrhoea
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DOI：
10.1099/jmm.0.049858-0
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发表时间：
2013-05-01
影响因子：
3
通讯作者：
Chen, Qing
中科院分区：
文献类型：
--
作者：
Feng, Jia-Li;Lin, Jin-Yan;Chen, Qing
香港海鸥形杆菌是一种潜在的新兴病原体，与社区获得性腹泻有关。为了流行病学的目的，不同的分子分型方法，如脉冲场凝胶电泳（PFGE）和多位点序列分型，已经开发了这种病原体。然而，这些方法需要专门的设备和昂贵的试剂。更重要的是，它们是劳动密集型和耗时的，这并不真正适合食源性疾病暴发的调查。在这项研究中，我们开发了一种快速，可靠的方法，使用22聚体特异性引物的肠细菌重复基因间共有序列（ERIC）。采用脉冲场凝胶电泳（PFGE）进行比较，对该方法进行评价。对81株L.从不同来源的食物中分离到79株，从香港人的病人中分离到2株。与香港人相关的感染。聚类分析表明，81株L.经ERIC-PCR和PFGE分析，hongkongensis菌株分别分为21和13个基因型。ERIC-PCR的重复性与PFGE相似。70.4%的菌株产生了可区分的ERIC-PCR图谱，这与PFGE结果一致。结果表明ERIC-PCR技术在L.香港花
Laribacter hongkongensis is a potential emerging pathogen, associated with community-acquired diarrhoea. For epidemiological purposes, different molecular typing methods, such as pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing, have been developed for this pathogen. However, these methods require specialized equipment and costly reagents. More importantly, they are labour-intensive and time-consuming, which is not really suitable for foodborne disease outbreak investigations. In this study, we developed a rapid and reliable method using 22-mer primers specific for the enterobacterial repetitive intergenic consensus sequence (ERIC). PFGE was used for comparison, to evaluate this method. A total of 81 isolates of L. hongkongensis were examined: 79 isolates recovered from food of diverse origins and two strains derived from patients with L. hongkongensis-associated infection. Typing patterns and clustering analysis indicated that the 81 L. hongkongensis isolates were grouped into 21 and 13 genotypes by ERIC-PCR and PFGE, respectively. ERIC-PCR was found as reproducible as PFGE. A high percentage (70.4%) of isolates yielded distinguishable ERIC-PCR patterns, which were concordant with the results from PFGE. These results suggest that ERIC-PCR is valuable for use in the epidemiological investigation of L. hongkongensis.