Immunolocalization of Myostatin (GDF-8) Following Musculoskeletal Injury and the Effects of Exogenous Myostatin on Muscle and Bone Healing
Immunolocalization of Myostatin (GDF-8) Following Musculoskeletal Injury and the Effects of Exogenous Myostatin on Muscle and Bone Healing
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DOI：
10.1369/0022155411425389
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Hamrick, Mark W.
中科院分区：
文献类型：
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作者：
Elkasrawy, Moataz;Immel, David;Hamrick, Mark W.
肌肉骨骼损伤后肌肉生长抑制素表达的时间过程和细胞定位尚不清楚;因此，作者在小鼠模型中评价了深部穿透性损伤后肌肉和骨修复期间肌肉生长抑制素的时间和空间定位。然后，他们在相同的损伤模型中使用外源性肌肉生长抑制素的水凝胶递送，以确定肌肉生长抑制素暴露对肌肉和骨骼愈合的影响。结果显示，在术后12-24小时，在受损的骨骼肌纤维中观察到强烈的肌生长抑制素染色的“池”，并且在截骨术后4天，肌生长抑制素也在软骨痂软骨细胞中表达。10或100 μ g/ml重组肌肉生长抑制素的水凝胶递送以剂量依赖性方式使骨折骨痂软骨面积相对于总骨痂面积减少41%和80%（p
The time course and cellular localization of myostatin expression following musculoskeletal injury are not well understood; therefore, the authors evaluated the temporal and spatial localization of myostatin during muscle and bone repair following deep penetrant injury in a mouse model. They then used hydrogel delivery of exogenous myostatin in the same injury model to determine the effects of myostatin exposure on muscle and bone healing. Results showed that a "pool" of intense myostatin staining was observed among injured skeletal muscle fibers 12-24 hr postsurgery and that myostatin was also expressed in the soft callus chondrocytes 4 days following osteotomy. Hydrogel delivery of 10 or 100 mu g/ml recombinant myostatin decreased fracture callus cartilage area relative to total callus area in a dose-dependent manner by 41% and 80% (p