Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes
Dissociation of porcine reproductive and respiratory syndrome virus neutralization from antibodies specific to major envelope protein surface epitopes
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DOI：
10.1016/j.virol.2012.08.026
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发表时间：
2012-11-25
期刊：
影响因子：
3.7
通讯作者：
Murtaugh, Michael P.
中科院分区：
文献类型：
--
作者：
Li, Juan;Murtaugh, Michael P.
猪繁殖与呼吸综合征病毒（PRRSV）的囊膜蛋白主要是糖蛋白5（GP 5）和膜蛋白（M）。虽然在2型PRRSV的GP 5和M中报道了病毒中和表位，但它们作为猪体液免疫靶点的意义还没有很好的描述。因此，我们构建了含有胞外域中和表位的重组多肽，以研究它们在猪抗体中和和抗病毒免疫中的作用。PRRSV感染引起胞外区特异性抗体，其滴度与中和抗体（NA）反应无关。来自PRRSV中和血清的胞外区特异性抗体结合病毒，但不中和感染性。此外，用胞外域多肽免疫猪产生特异性抗体，并提供部分保护，而没有可检测到的NA应答。最后，多肽不阻断猪巨噬细胞的感染。这些结果表明，GP 5/M胞外域肽表位可用于宿主抗体识别，但与抗体介导的病毒中和无关。(C)2012 Elsevier Inc. All rights reserved.
Glycoprotein 5 (GP5) and membrane (M) protein are the major proteins in the envelope of porcine reproductive and respiratory syndrome virus (PRRSV). Although viral neutralization epitopes are reported in GP5 and M of type 2 PRRSV, their significance as targets of porcine humoral immunity is not well described. Thus, we constructed recombinant polypeptides containing ectodomain neutralization epitopes to examine their involvement in porcine antibody neutralization and antiviral immunity. PRRSV infection elicited ectodomain-specific antibodies, whose titers did not correlate with the neutralizing antibody (NA) response. Ectodomain-specific antibodies from PRRSV-neutralizing serum bound virus but did not neutralize infectivity. Furthermore, immunization of pigs with ectodomain polypeptides raised specific antibodies and provided partial protection without a detectable NA response. Finally the polypeptides did not block infection of porcine macrophages. These results suggest that the GP5/M ectodomain peptide epitopes are accessible for host antibody recognition, but are not associated with antibody-mediated virus neutralization. (C) 2012 Elsevier Inc. All rights reserved.