A human fibrosarcoma cell line producing multiplication stimulating activity (MSA)-related peptides
A human fibrosarcoma cell line producing multiplication stimulating activity (MSA)-related peptides
复制标题
产生增殖刺激活性（MSA）相关肽的人纤维肉瘤细胞系
作者：
J. D. Larco;G. Todaro
我们以前建议，转化细胞可能会产生和释放细胞生长因子，能够与特定类别的细胞受体相互作用，使它们无法作为外部配体的受体1，2。这个模型的预测是，一个人纤维肉瘤线，已被证明不结合125碘标记的增殖刺激活性（MSA）2，可能分泌分子功能相关的MSA。我们在这里描述的生长因子产生的这种人类肿瘤细胞系，来自一个25岁的女性与纤维肉瘤的腿的一些属性。这些细胞产生一系列具有不同分子量的MSA相关化合物，这些化合物可以在Sephadex G-75上分离。主要活性对应于分子量（MW）约为11，000和7，000的蛋白质。这两种组分都刺激细胞分裂，并将与125 I标记的MSA竞争其在小鼠、大鼠或人细胞膜上的受体。培养的纤维肉瘤细胞可能构成人血清和血浆的重要替代来源，用于生长刺激因子的分离和表征。
WE suggested previously that transformed cells may produce and release cellular growth factors capable of interacting with a specific class of cellular receptors, making them unavailable as receptors for external ligands1,2. A prediction of this model was that a human fibrosarcoma line, which had been shown not to bind 125I-labelled multiplication stimulating activity (MSA)2, might be secreting molecules functionally related to MSA. We describe here some properties of growth factors produced by this human tumour cell line, derived from a 25-yr-old female with fibrosarcoma of the leg. The cells produce a family of MSA-related compounds with different molecular weights that can be separated on Sephadex G-75. The major activities correspond to proteins of approximate molecular weight (MW) of 11,000 and 7,000. Both of these fractions stimulate cell division, and will compete with 125I-labelled MSA for its receptors on mouse, rat or human cell membranes. Fibrosarcoma cells in culture may constitute an important alternate source to human serum and plasma for the isolation and characterisation of growth stimulating factors.