Androgen Receptor as a Potential Sign of Prostate Cancer Metastasis
Androgen Receptor as a Potential Sign of Prostate Cancer Metastasis
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DOI：
10.1002/pros.21021
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发表时间：
2009-11-01
期刊：
影响因子：
2.8
通讯作者：
Labrie, Fernand
中科院分区：
文献类型：
--
作者：
Levesque, Marie-Helene;El-Alfy, Mohamed;Labrie, Fernand
背景资料。雄激素受体(AR)的表达及其在癌变过程中的调节已经在几个研究中被研究过，但结果相互矛盾。应用原位杂交和免疫细胞化学方法检测良性、高级别前列腺上皮内瘤变(HGPIN)和前列腺癌组织中AR的表达。结果：与良性组织相比，前列腺癌组织中AR基因的表达水平显著升高。HGPIN中的AR丰度被发现几乎是良性组织和癌组织中观察到的丰度的一半。在良性前列腺上皮中，免疫细胞化学数据显示AR仅在上皮细胞的细胞核中表达。然而，在被检查的癌活检组织中，72%的AR在细胞质和细胞核中都有表达。在查阅了100例前列腺癌患者的病历后发现，81%的前列腺癌患者在前列腺癌向外转移时，AR在癌细胞的两个细胞室都有表达。相比之下，当癌症局限于器官时，仅66%的病例AR位于细胞核和细胞质中。此外，当AR表达于癌细胞的胞浆中时，连续切片显示AR定位于线粒体。结论：前列腺癌组织中AR的表达明显高于良性前列腺组织。前列腺69：1704-1711,2009。(C)2009年Wiley-Liss，Inc.
BACKGROUND. Androgen receptor (AR) expression and its modulation through the carcinogenesis process have been investigated in several studies with conflicting results.MATERIALS AND METHODS. In situ hybridization and immunocytochemistry were used to examine AR expression in prostatic needle core biopsies of benign, high grade prostatic intraepithelial neoplasia (HGPIN) and prostatic adenocarcinoma.RESULTS. A significant increase in AR mRNA levels was found in the cancerous prostatic cells when compared with the benign tissue biopsies. AR abundance in HGPIN was found to be almost half-way between that observed in benign and in cancerous tissue. In the benign prostatic epithelium, the immunocytochemistry data show that AR is exclusively expressed in the nuclei of epithelial cells. However, in 72% of examined cancer biopsies, AR was expressed in both the cytoplasm and nuclei. After examination of medical records of 100 patients diagnosed with prostate cancer, it was found that the AR was expressed in both cellular compartments of cancer cells in 81% of cases when cancer was found to have metastasized outside the prostate. In contrast, when the cancer was organ-confined, AR was localized in both the nuclei and cytoplasm in only 66% of cases. Moreover, when the AR was expressed in the cytoplasm of cancerous cells, consecutive serial sections immunostained with the mitochondrial marker suggest that AR is localized in the mitochondria.CONCLUSIONS. AR mRNA expression is significantly higher in prostate cancer when compared to benign prostatic tissue. Prostate 69: 1704-1711, 2009. (C) 2009 Wiley-Liss, Inc.