Expression of transforming growth factor alpha (TGF-alpha) gene in mouse embryonic development.
Expression of transforming growth factor alpha (TGF-alpha) gene in mouse embryonic development.
复制标题
转化生长因子α（TGF-α）基因在小鼠胚胎发育中的表达。
DOI：
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发表时间：
1997
影响因子：
3.1
通讯作者：
N. Terakawa
中科院分区：
文献类型：
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作者：
T. Harada;T. Fujikawa;S. Yoshida;Y. Onohara;M. Tanikawa;N. Terakawa
目的
通过逆转录-聚合酶链反应（RT-PCR）评价小鼠囊胚中TGF-α、表皮生长因子（EGF）和EGF受体（EGFR）基因的表达。本研究探讨了TGF-α和EGF对小鼠胚胎着床前发育的影响。
结果
结果显示存在TGF-α和EGFR的转录物。但在重复实验中未观察到EGF mRNA。当添加到培养基中时，这些生长因子都不影响从两细胞期到胚泡期的发育速率。这些影响进一步检查测量掺入的氚化胸苷和亮氨酸，分别提供DNA和蛋白质的合成的指数。浓度仅为0.1 ng/ml的TGF-α与EGF共享细胞表面受体，刺激DNA和蛋白质的合成。EGF在10 ng/ml的浓度刺激DNA和蛋白质的囊胚的合成。为了探索TGF-α对囊胚腔扩张速率的自分泌作用，使用TGF-α反义寡脱氧核苷酸来降低TGF-α基因的表达。浓度为0.1 ng/ml的TGF-α刺激早期空化小鼠囊胚中囊胚腔扩张的速率。相反，TGF-α反义寡核苷酸显著降低了扩增速率。
结论
我们目前的观察表明，TGF-α/EGF和EGFR可能参与调节胚胎发育。特别是，TGF-α可以作为自分泌因子调节胚胎发育。
PURPOSE
The expression of genes for TGF-alpha, epidermal growth factor (EGF), and the EGF receptor (EGFR) in mouse blastocysts was evaluated by the reverse transcription-polymerase chain reaction (RT-PCR). We evaluated the effects of TGF-alpha and EGF on the development of mouse embryo prior to implantation.
RESULTS
The results revealed the presence of transcripts of TGF-alpha and EGFR. However, EGF mRNA was not observed in repeated experiments. None of these growth factors influenced the rate of development from the two-cell stage to the blastocyst stage when added to the culture medium. These effects were further examined on measuring the incorporation of tritiated thymidine and leucine, providing indices of the synthesis of DNA and protein, respectively. A concentration of only 0.1 ng/ml of TGF-alpha, which shares a cell surface receptor with EGF, stimulated the synthesis of both DNA and protein. EGF at a concentration of 10 ng/ml stimulated the synthesis of DNA and protein by blastocysts. To explore autocrine effects of TGF-alpha on the rate of blastocoel expansion, TGF-alpha antisense oligodeoxynucleotides was used to reduce expression of the TGF-alpha gene. TGF-alpha at a concentration of 0.1 ng/ml stimulates the rate of blastocoel expansion in early cavitating mouse blastocysts. In contrast, TGF-alpha antisense oligonucleotides significantly reduced the rate of expansion.
CONCLUSIONS
Our present observations suggest that TGF-alpha/EGF and the EGFR may be involved in regulating embryonic development. In particular, TGF-alpha may serve as an autocrine factor in the regulation of embryonic development.