CYCLING OF CALCIUM, SODIUM, AND PROTONS ACROSS INNER MEMBRANE OF CARDIAC MITOCHONDRIA
CYCLING OF CALCIUM, SODIUM, AND PROTONS ACROSS INNER MEMBRANE OF CARDIAC MITOCHONDRIA
复制标题
DOI：
10.1111/j.1432-1033.1978.tb12713.x
复制
发表时间：
1978-01-01
期刊：
影响因子：
--
通讯作者：
HEID, I
中科院分区：
文献类型：
--
作者：
CROMPTON, M;HEID, I
描述了一种方法，允许同时测定的净电荷转移与钙离子运输的Ru-红敏感的载体和电离的内部[Ca 2 +]在大鼠心脏线粒体。该载体催化Ca 2+的电荷未补偿通量。对Ca 2+内流的完全电荷补偿由H+的呼吸依赖性流出提供。Na+诱导的Ca ~（2+）净流出是根据另外两种载体（Na ~+-Ca ~（2+）反向转运体和Na ~+-H ~+反向转运体）来分析的。这两种载体可能是分离的，Na+-H+交换更快。在Na+诱导的Ca 2+流出过程中，Ca 2+、Na+和H+的通量支持Na+-H+交换事件，其中Na+-H+交换使得单向Ca 2+通量能够通过单向和反向系统整合到一个循环中。
A method is described that permits simultaneous determination of the net charge transfer associated with Ca2+ transport by the Ru-red-sensitive carrier and ionized internal [Ca2+] in rat heart mitochondria. This carrier catalyzes a charge-uncompensated flux of Ca2+. Full charge compensation for Ca2+ influx is provided by the respiration-dependent efflux of H+. The net efflux of Ca2+ induced by Na+ is analyzed in terms of 2 other carriers, a Na+-Ca2+ antiporter and a Na+-H+ antiporter. These 2 carriers are probably separate and Na+-H+ exchange is more rapid. The fluxes of Ca2+, Na+ and H+ during the Na+-induced efflux of Ca2+ support events where Na+-H+ exchange enables unidirectional Ca2+ fluxes via the uniport and antiport systems to be integrated into a cycle.