Synthesis and comparative study on the antimicrobial activity of hybrid materials based on silver nanoparticles (AgNps) stabilized by polyvinylpyrrolidone (PVP).
Synthesis and comparative study on the antimicrobial activity of hybrid materials based on silver nanoparticles (AgNps) stabilized by polyvinylpyrrolidone (PVP).
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DOI：
10.1007/s12154-011-0063-9
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发表时间：
2011-10-01
期刊：
影响因子：
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通讯作者：
Kantardjiev, Todor
中科院分区：
文献类型：
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作者：
Bryaskova, Rayna;Pencheva, Daniela;Kantardjiev, Todor
以聚乙烯吡咯烷酮（PVP）为稳定剂，采用热还原法和化学还原法合成了聚乙烯吡咯烷酮/银纳米粒子杂化材料。TEM分析证实了球形银纳米粒子的形成，其直径在9 ~ 16 nm之间。紫外-可见光谱和红外光谱也被应用于确认AgNps的成功形成。合成的AgNP/PVP对三组不同细菌的标准菌株-金黄色葡萄球菌（S. aureus）的抗菌活性。金黄色葡萄球菌;革兰氏阳性菌）、大肠杆菌（E.大肠杆菌属;革兰氏阴性菌）、铜绿假单胞菌（P. aeruginosa;非发酵革兰氏阴性菌）以及枯草芽孢杆菌（B. subtilis）进行了研究。测试AgNps/PVP对不同酵母和霉菌如白色念珠菌、克鲁斯念珠菌、热带念珠菌、光滑念珠菌和巴西曲霉的杀真菌活性的存在。该杂化材料对测试的细菌和真菌菌株显示出很强的抗菌作用，因此在生物技术和生物医学科学中具有潜在的应用。
Hybrid materials based on polyvinylpyrrolidone (PVP) with silver nanoparticles (AgNps) were synthesized applying two different strategies based on thermal or chemical reduction of silver ions to silver nanoparticles using PVP as a stabilizer. The formation of spherical silver nanoparticles with diameter ranging from 9 to 16nm was confirmed by TEM analysis. UV-vis and FTIR spectroscopy were also applied to confirm the successful formation of AgNps. The antibacterial activity of the synthesized AgNPs/PVP against etalon strains of three different groups of bacteria-Staphylococcus aureus (S. aureus; gram-positive bacteria), Escherichia coli (E. coli; gram-negative bacteria), Pseudomonas aeruginosa (P. aeruginosa; non-ferment gram-negative bacteria), as well as against spores of Bacillus subtilis (B. subtilis) was studied. AgNps/PVP were tested for the presence of fungicidal activity against different yeasts and mold such as Candida albicans, Candida krusei, Candida tropicalis, Candida glabrata, and Aspergillus brasiliensis. The hybrid materials showed a strong antimicrobial effect against the tested bacterial and fungal strains and therefore have potential applications in biotechnology and biomedical science.