Embryonic intraventricular exposure to autism-specific maternal autoantibodies produces alterations in autistic-like stereotypical behaviors in offspring mice.
Embryonic intraventricular exposure to autism-specific maternal autoantibodies produces alterations in autistic-like stereotypical behaviors in offspring mice.
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DOI：
10.1016/j.bbr.2014.02.045
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发表时间：
2014-06-01
影响因子：
2.7
通讯作者：
Martínez-Cerdeño V
中科院分区：
文献类型：
--
作者：
Camacho J;Jones K;Miller E;Ariza J;Noctor S;de Water JV;Martínez-Cerdeño V
多项研究表明，母体自身抗体对自闭症谱系障碍（ASD）的病因学中的胎儿脑蛋白特异性反应的作用。在目前的研究中，我们研究了自闭症儿童（MAU）母亲的脑反应性母体自身抗体对小鼠后代行为的影响，与暴露于典型发育儿童（MTD）母亲的非反应性IgG的后代相比。在胚胎第14天，将胚胎后代暴露于单次脑室内注射MAU或MTD IgG。后代被允许成熟到成年，随后测试社交能力和刻板行为，使用3室社会方法任务，大理石埋葬任务，并评估自发梳理行为，以应对新的环境。结果表明，MAU后代在埋葬大理石和自发梳理行为中都表现出类似自闭症的刻板行为。此外，与MTD后代相比，在MAU后代中也观察到社会接近行为的微小变化。本报告首次证明了来自单个MAU样本的IgG单次低剂量脑室内暴露对后代行为的影响。
Multiple studies have implicated a role of maternal autoantibodies reactive against fetal brain proteins specific to autism in the etiology of autism spectrum disorders (ASD). In the current study, we examined the impact of brain-reactive maternal autoantibodies of mothers of children with autism (MAU) on offspring behavior in mice compared to offspring exposed to non-reactive IgG of mothers of typically developing children (MTD). Embryonic offspring were exposed to a single intraventricular injection of MAU or MTD IgG on embryonic day 14. Offspring were allowed to mature to adulthood and were subsequently tested for sociability and stereotypic behaviors using a 3-chambered social approach task, marble burying task, and assessment of spontaneous grooming behaviors in response to a novel environment. Results indicate that MAU offspring display autistic-like stereotypic behavior in both marble burying and spontaneous grooming behaviors. Additionally, small alterations in social approach behavior were also observed in MAU offspring compared to MTD offspring. This report demonstrates for the first time the effects of a single, low dose intraventricular exposure of IgG derived from individual MAU samples on offspring behavior.