Monocytes and macrophages synthesize and secrete thrombospondin.
Monocytes and macrophages synthesize and secrete thrombospondin.
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DOI：
10.1182/blood.v65.1.79.bloodjournal65179
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发表时间：
1985
期刊：
影响因子：
20.3
通讯作者：
E. Jaffe;J. T. Ruggiero;D. Falcone
中科院分区：
文献类型：
--
作者：
E. Jaffe;J. T. Ruggiero;D. Falcone
凝血酶敏感蛋白是由凝血酶刺激的血小板α颗粒释放的主要糖蛋白之一，是一种二硫键连接的160，000-道尔顿亚基的三聚体。培养的人单核细胞以时间依赖性方式（1.45微克/10（6）个细胞/24小时）将血小板反应蛋白（通过酶联免疫吸附试验测定）分泌到培养基中;分泌完全被放线菌酮（1微克/mL）阻断。用兔抗血小板反应蛋白偶联蛋白A-Sepharose，从35 S-蛋氨酸标记的人单核细胞培养后的培养基中分离35 S-血小板反应蛋白。免疫分离的35 S-血小板反应蛋白迁移在十二烷基硫酸钠-聚丙烯酰胺凝胶还原后的分子量为159，000。使用小鼠驻留的腹膜巨噬细胞获得了类似的结果。从用内毒素、酪蛋白或巯基乙酸盐预处理的小鼠收获的引发性腹腔巨噬细胞分泌的血小板反应蛋白比从对照小鼠收获的常驻巨噬细胞少得多。因此，来自两个不同物种的单核细胞和巨噬细胞合成并分泌血小板反应蛋白，并且血小板反应蛋白的合成速率似乎取决于细胞的活化状态。
Thrombospondin, one of the major glycoproteins released from alpha-granules of thrombin-stimulated platelets, is a disulfide-linked trimer of 160,000-dalton subunits. Cultured human monocytes secreted thrombospondin (determined by an enzyme-linked immunosorbent assay) into the culture medium in a time-dependent manner (1.45 micrograms/10(6) cells/24 hr); secretion was totally blocked by cycloheximide (1 microgram/mL). 35S-thrombospondin was isolated from 35S-methionine-labeled human monocyte postculture medium with rabbit polyclonal anti-thrombospondin coupled to protein A-Sepharose. The immunoisolated 35S-thrombospondin migrated in sodium dodecyl sulfate-polyacrylamide gels after reduction with a molecular weight of 159,000. Similar results were obtained using mouse resident peritoneal macrophages. Elicited peritoneal macrophages harvested from mice pretreated with endotoxin, casein, or thioglycollate secreted much less thrombospondin than did resident macrophages harvested from control mice. Thus, monocytes and macrophages from two different species synthesize and secrete thrombospondin, and the rate of synthesis of thrombospondin appears to depend on the state of activation of the cells.