Analgesic effects of intrathecal curcumin in the rat formalin test.
Analgesic effects of intrathecal curcumin in the rat formalin test.
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DOI：
10.3344/kjp.2012.25.1.1
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Kim WM
中科院分区：
文献类型：
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作者：
Han YK;Lee SH;Jeong HJ;Kim MS;Yoon MH;Kim WM
据报道，全身给药时，姜黄素具有抗炎、抗氧化、抗病毒、抗真菌、抗肿瘤和抗伤害作用。我们研究了鞘内注射姜黄素对炎性疼痛大鼠的镇痛效果。雄性SD大鼠做鞘内插管准备。后爪注射5%福尔马林溶液(50微克L)诱发疼痛。通过鞘内导管分别给予62.5、125、250和500微克的姜黄素以检查退缩反应。用对数尺度上的最小二乘线性回归拟合剂量-反应曲线，计算出姜黄素在福尔马林试验两个阶段的ED50值(半数有效剂量)和95%可信区间。鞘内注射姜黄素的大鼠，缩腿反应在两个时相均显著降低。姜黄素的ED50值(95%可信区间)为511.4µg(23.5~1126.5)。姜黄素给药后未见明显异常行为。鞘内注射姜黄素可减轻大鼠炎性疼痛，有必要进一步研究姜黄素脊髓作用的确切机制。
Curcumin has been reported to have anti-inflammatory, antioxidant, antiviral, antifungal, antitumor, and antinociceptive activity when administered systemically. We investigated the analgesic efficacy of intrathecal curcumin in a rat model of inflammatory pain. Male Sprague Dawley rats were prepared for intrathecal catheterization. Pain was evoked by injection of formalin solution (5%, 50 µl) into the hind paw. Curcumin doses of 62.5, 125, 250, and 500 µg were delivered through an intrathecal catheter to examine the flinching responses. The ED50 values (half-maximal effective dose) with 95% confidence intervals of curcumin for both phases of the formalin test were calculated from the dose-response lines fitted by least-squares linear regression on a log scale. In rats with intrathecal administration of curcumin, the flinching responses were significantly decreased in both phases. The slope of the regression line was significantly different from zero only in phase 2, and the ED50 value (95% confidence interval) of curcumin was 511.4 µg (23.5-1126.5). There was no apparent abnormal behavior following the administration of curcumin. Intrathecal administration of curcumin decreased inflammatory pain in rats, and further investigation to elucidate the precise mechanism of spinal action of curcumin is warranted.