Uptake and transformation of phenol and chlorophenols by hairy root cultures of Daucus carota, Ipomoea batatas and Solanum aviculare.
Uptake and transformation of phenol and chlorophenols by hairy root cultures of Daucus carota, Ipomoea batatas and Solanum aviculare.
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DOI：
10.1016/j.chemosphere.2005.08.005
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发表时间：
2006-04
期刊：
影响因子：
8.8
通讯作者：
B. S. de Araujo;J. Dec;J. Bollag;M. Pletsch
中科院分区：
文献类型：
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作者：
B. S. de Araujo;J. Dec;J. Bollag;M. Pletsch
胡萝卜毛状根培养物，番薯和茄（Solanum aviculare Forst）对高毒性污染物苯酚和氯酚的敏感性以及固有的过氧化物酶参与从液体介质中去除苯酚的情况进行了研究。根D。胡萝卜在含1000μmoll− 1苯酚的培养基中生长正常，而I. batatas和S.只有在500μmoll−1的水平下才有可能出现扁形虫。在氯酚存在的情况下，只有在浓度不超过50μ mol −1时，根才可能正常生长，但I除外。在所有浓度下都受到严重影响的甘薯。尽管生物量减少，但S.在含有高达2000μ mol − 1苯酚或2-氯苯酚和高达500μ mol − 1 2，6-二氯苯酚的培养基中，Aviculare培养物得以维持。处理72 h内，根对苯酚的去除量分别为初始浓度的72.7%、90.7%和98.6%;卡罗塔岛batatas和S. aviculare，分别。对2，6-二氯苯酚的去除率分别为83.0%、57.7%和73.1%。~（14）C标记的酚类物质被根组织吸收，与高极性的细胞物质凝聚，并结合到细胞壁或细胞膜中。结果表明，S.观赏植物扁蓄最适合在田间条件下进行补救试验。
Hairy root cultures of Daucus carota L., Ipomoea batatas L. and Solanum aviculare Forst were investigated for their susceptibility to the highly toxic pollutants phenol and chlorophenols and for the involvement of inherent peroxidases in the removal of phenols from liquid media. Roots of D. carota grew normally in medium containing 1000μmoll−1of phenol, whilst normal growth of roots of I. batatas and S. aviculare was only possible at levels up to 500μmoll−1. In the presence of chlorophenols, normal root growth was possible only in concentrations not exceeding 50μmoll−1, except for I. batatas which was severely affected at all concentrations. Despite the reduction in biomass, the growth of S. aviculare cultures was sustained in medium containing up to 2000μmoll−1of phenol or 2-chlorophenol, and up to 500μmoll−1of 2,6-dichlorophenol. The amounts of phenol removed by the roots within 72h of treatment were 72.7%, 90.7% and 98.6% of the initial concentration for D. carota, I. batatas and S. aviculare, respectively. For the removal of 2,6-dichlorophenol the values were, respectively, 83.0%, 57.7% and 73.1%. Phenols labelled with14C were absorbed by the root tissues and condensed with highly polar cellular substances as well as being incorporated into the cell walls or membranes. The results suggest that S. aviculare, an ornamental plant, would be best suited for remediation trials under field conditions.