Production of two morphologically different antimony trioxides by a novel antimonate-reducing bacterium, Geobacter sp. SVR
Production of two morphologically different antimony trioxides by a novel antimonate-reducing bacterium, Geobacter sp. SVR
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DOI：
10.1016/j.jhazmat.2021.125100
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发表时间：
2021-01-22
影响因子：
13.6
通讯作者：
Amachi, Seigo
中科院分区：
文献类型：
--
作者：
Yamamura, Shigeki;Iida, Chisato;Amachi, Seigo
从原锑矿土壤中分离到一株新型异化锑酸盐还原菌SVR。菌株SVR将Sb（V）还原与乙酸盐氧化偶联，表观还原速率为2.4 mM d-1。Sb（V）的还原伴随着白色微晶在液体介质中的沉淀和积累。沉淀物最初是小的和无定形的，但他们最终发展到晶体相的长度> 50？M.菌株SVR去除96%的溶解的Sb作为沉淀物。X射线衍射分析表明，微晶是正交晶系的三氧化二锑（Sb 2 O3），即，钒钛矿系统发育分析和生理生化分析表明，该菌株为Geophylla属的成员。在pH7.0条件下，乙酸盐和Sb（V）对SVR菌株的细胞悬浮液进行培养，可形成紫轮藻。有趣的是，在pH 8.0时，细胞悬浮液形成另一种具有立方结构的晶体Sb 2 O 3，即，方铅矿我们的研究结果提供了直接的证据，Geophysics spp。在自然界中参与Sb（V）还原。该菌株具有良好的上级除锑能力，可用于含锑废水中锑的回收以及单独生产钒钛矿和方锑矿。
A novel dissimilatory antimonate [Sb(V)]-reducing bacterium, strain SVR, was isolated from soil of a former antimony (Sb) mine. Strain SVR coupled Sb(V) reduction to acetate oxidation with an apparent reduction rate of 2.4 mM d-1. The reduction of Sb(V) was followed by the precipitation and accumulation of white microcrystals in the liquid medium. The precipitates were initially small and amorphous, but they eventually developed to the crystal phase with a length > 50 ?m. Strain SVR removed 96% of dissolved Sb as the precipitates. An X-ray diffraction analysis indicated that the microcrystals were the orthorhombic Sb trioxide (Sb2O3), i.e., valentinite. Phylogenetic and physiological analyses revealed that strain SVR is a member of the genus Geobacter. The cell suspension of strain SVR incubated with acetate and Sb(V) at pH 7.0 was able to form valentinite. Interestingly, at pH 8.0, the cell suspension formed another crystalline Sb2O3 with a cubic structure, i.e., senarmontite. Our findings provide direct evidence that Geobacter spp. are involved in Sb(V) reduction in nature. Considering its superior capacity for Sb removal, strain SVR could be used for the recovery of Sb and the individual productions of valentinite and senarmontite from Sb-contaminated wastewater.