In vivo imaging of dendritic pruning in dentate granule cells.
In vivo imaging of dendritic pruning in dentate granule cells.
复制标题
齿状颗粒细胞中树突状修剪的体内成像。
DOI：
10.1038/nn.4301
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发表时间：
2016-06
影响因子：
25
通讯作者：
Gage FH
中科院分区：
文献类型：
--
作者：
Gonçalves JT;Bloyd CW;Shtrahman M;Johnston ST;Schafer ST;Parylak SL;Tran T;Chang T;Gage FH
我们在体内对成年出生的小鼠齿状颗粒细胞(DGC)发育中的树突进行了纵向成像，发现它们经历了过度分枝和修剪。暴露于丰富的环境(EE)和通过干扰Wnt信号抑制树枝生长导致了增加的分枝增加和加速的生长，然而，这被更早和更广泛的修剪所抵消。我们的结果表明，修剪是以一种动态平衡的方式调节的，以反对过度的分枝，并促进DGC中类似的树突结构。
We longitudinally imaged the developing dendrites of adult-born mouse dentate granule cells (DGCs) in vivo and found that they underwent over-branching and pruning. Exposure to an enriched environment (EE) and constraining dendritic growth by disrupting Wnt signaling led to increased branch addition and accelerated growth, which were, however, counteracted by earlier and more extensive pruning. Our results indicate that pruning is regulated in a homeostatic fashion to oppose excessive branching and promote a similar dendrite structure in DGCs.