Reduced function of endothelial nitric oxide and hyperpolarization in artery grafts with poor runoff
Reduced function of endothelial nitric oxide and hyperpolarization in artery grafts with poor runoff
复制标题
径流不良的移植动脉内皮一氧化氮功能降低和超极化
DOI：
10.1016/j.jss.2021.08.031
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发表时间：
2022
期刊：
影响因子：
2.2
通讯作者：
and Takeo Itoh.
中科院分区：
文献类型：
--
作者：
Masashi Sakakibara;Akio Kodama;Kimihiro Komori;and Takeo Itoh.
研究背景内皮细胞通过释放一氧化氮（endothelium-衍生的一氧化氮，EDNO）和超极化因子（endothelium-衍生的超极化因子，EDHF）来调节血管紧张度。在径流量差的静脉移植物中，这些因素功能的缺乏会导致严重的内膜增生。本研究评估了在不良径流条件下EDNO和EDHF在动脉移植物中的功能如何改变。 材料和方法在正常径流条件（“非闭塞移植物”）或不良径流条件（“差径流移植物”）下，切除兔右侧颈总动脉并作为自体移植物植入其原始位置。检查了组织化学变化、乙酰胆碱 (ACh) 诱导的内皮依赖性舒张作用和平滑肌细胞 (SMC) 超极化。结果两种动脉移植物类型均显示出可忽略不计的内膜增生。在存在或不存在 EDNO 合酶抑制剂的情况下，径流较差的移植物中乙酰胆碱诱导的松弛程度低于非闭塞移植物。此外，在径流较差的移植物组中，ACh诱导的而不是非受体激动剂A23187诱导的SMC超极化低于非闭塞移植物组。结论与静脉移植物不同，自体颈动脉移植物在径流条件差的情况下EDNO和EDHF的功能降低，但部分维持。在这样的动脉移植物中，不存在由外科手术引起的内膜增生。这些结果可以解释动脉移植物与静脉移植物相比通畅性改善的一些机制。
BackgroundThe endothelium regulates vascular tonus by releasing nitric oxide (endothelium-derived nitric oxide, EDNO) and hyperpolarizing factor (endothelium-derived hyperpolarizing factor, EDHF). In vein grafts with poor runoff, lack of function of these factors causes severe intimal hyperplasia. This study evaluated how the functions of EDNO and EDHF are altered in artery grafts under poor runoff conditions.Materials and MethodsThe right common carotid arteries of rabbits were excised and implanted in their original positions as autogenous grafts under normal runoff conditions (“nonoccluded grafts”) or poor runoff conditions (“poor runoff grafts”). Histochemical changes, acetylcholine (ACh)-induced effects on endothelium-dependent relaxation and smooth muscle cell (SMC) hyperpolarization were examined.ResultsBoth artery graft types displayed negligible intimal hyperplasia. In the absence and presence of an EDNO synthase inhibitor, ACh-induced relaxation was lower in grafts with poor runoff than in nonoccluded grafts. Furthermore, ACh-induced but not nonreceptor agonist A23187-induced SMC hyperpolarization was lower in the poor runoff graft group than in the nonoccluded graft group.ConclusionsUnlike in those in vein grafts, the functions of EDNO and EDHF in autogenous carotid artery grafts under poor runoff conditions were reduced but partly maintained. In such artery grafts, intimal hyperplasia caused by surgical operation was not present. These results may explain some of the mechanisms underlying the improved patency of artery grafts compared with vein grafts.