A new ganglioside in human meconium detected with antiserum against human milk sialyltetrasaccharide a.
A new ganglioside in human meconium detected with antiserum against human milk sialyltetrasaccharide a.
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用抗人乳唾液酸四糖a的抗血清检测到人胎粪中的新神经节苷脂。
DOI：
10.1016/0003-9861(86)90580-1
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发表时间：
1986
影响因子：
3.9
通讯作者：
Smith,DF
中科院分区：
文献类型：
--
作者：
Prieto,PA;Smith,DF
针对人乳单唾液低聚糖唾液酸四糖的糖醇衍生物的抗血清[D.F.Smith，P.A.Prieto，和B.V.Torres(1985)Arch.生物化学。BiPhys.241,298-303]通过将神经节苷脂臭氧分解和碱解后得到的唾液酸寡糖糖苷直接结合到硝酸纤维滤膜上，检测到人胎粪中的一种新的神经节苷脂。唾液酸寡糖通过含有抗唾液酸四糖抗体的柱亲和层析纯化。亲和纯化的唾液酸寡糖与从母乳中提取的唾液酸四糖的亲和标记的阿迪醇衍生物共层析。纯唾液酸寡糖的连续酶降解和消化产物与标准共层析的结果与胎粪中存在的具有NeuAcα2–3Galβ1–3GlcNAcβ1–3Galβ1–4Glc-ceramide.结构的单唾液神经节苷脂一致该神经节苷脂可能是唾液酸脂的生物合成前体[G.C.Hansson和D.Zopf(1985)J.Biol]。化学260,9388-9392]，它也是人胎粪的一种成分。
Antiserum directed against the alditol derivative of the human milk monosialyloligosaccharide sialyltetrasaccharidea[D. F. Smith, P. A. Prieto, and B. V. Torres (1985)Arch. Biochem. Biophys.241, 298–303] is used to detect a new ganglioside in human meconium by direct binding on nitrocellulose filters of the sialyl[3H]oligosaccharide alditol obtained from gangliosides after ozonolysis and alkali fragmentation. The sialyl[3H]oligosaccharide is purified by affinity chromatography on a column containing anti-sialyltetrasaccharideaantibodies. The affinity-purified sialyl[3H]oligosaccharide cochromatographs with the3H-labeled alditol derivative of authentic sialyltetrasaccharideafrom human milk. Results of sequential enzyme degradation of the pure sialyl[3H]oligosaccharide and cochromatography of the digestion products with standards are consistent with the presence in meconium of a monosialylganglioside with the structure NeuAcα2–3Galβ1–3GlcNAcβ1–3Galβ1–4Glc-ceramide. This ganglioside is presumably the biosynthetic precursor of the sialyl-Leaganglioside [G. C. Hansson and D. Zopf (1985)J. Biol. Chem.260, 9388–9392], which is also a component of human meconium.